Characteristics and function of T cells in tumor-draining lymph nodes of colorectal cancer
DOI：10.3872/j.issn.1007-385x.2021.09.002
- VernacularTitle:结直肠癌肿瘤引流淋巴结T细胞的特征及其功能
- Author:
TANG Jinglinga
1
;
YANG Yuanb
2
;
LI Xiaoyunc
3
;
DU Hangb
4
;
WANG Xinjunb
2
;
LIU Yanjiea
5
Author Information
1. a. Department of Pathology, Clinical Medical College, the Affiliated Hospital of Guizhou Medical University, Guiyang 550004, Guizhou, China
2. b. Clinical Medical Research Center, the Affiliated Hospital of Guizhou Medical University, Guiyang 550004, Guizhou, China
3. c. Department of Anorectal Surgery, the Affiliated Hospital of Guizhou Medical University, Guiyang 550004, Guizhou, China
4. b. Clinical Medical Research Center, the Affiliated Hospital of Guizhou Medical University, Guiyang 550004, Guizhou, China
5. a. Department of Pathology, Clinical Medical College, the Affiliated Hospital of Guizhou Medical University, Guiyang 550004, Guizhou, China
- Publication Type:Journal Article
- Keywords:
结直肠癌；肿瘤引流淋巴结；免疫微环境；T细胞活化；T细胞抑制
- From:
Chinese Journal of Cancer Biotherapy
2021;28(9):869-876
- CountryChina
- Language:Chinese
-
Abstract:
[摘 要] 目的：探讨结直肠癌（colorectal cancer，CRC）肿瘤引流淋巴结（tumor-draining lymph node，TDLN）中T细胞的免疫学特性及其抗肿瘤作用。方法：收集2018年12月至2021年1月贵州医科大学附属医院收治的33例CRC患者的临床资料和淋巴结标本。采用染料法示踪，配对采集CRC患者TDLN和非肿瘤引流淋巴结（non-TDLN，NTDLN）。用流式细胞术检测已制备成单细胞悬液的TDLN和NTDLN中免疫细胞亚群和功能表型差异，酶联免疫斑点法比较TDLN和NTDLN中肿瘤反应性T细胞比例，多色免疫荧光组织化学技术分析两种淋巴结中免疫细胞空间分布情况。体外扩增TDLN-T细胞，检测其T细胞亚群和表型变化以及肿瘤免疫反应能力。结果：与NTDLN相比，TDLN中含有更高比例肿瘤反应性T细胞（P<0.05），调节性T（Treg）细胞比例较高（P<0.01），但单核样髓源性抑制细胞比例较低（P<0.05），T细胞活化标志物ICOS、CD28和抑制标志物PD-1、TIGIT比例均显著升高（P<0.05或P<0.01）。Treg细胞和滤泡性T细胞主要分布在淋巴结皮质区和生发中心。TDLN-T细胞扩增后，以CD8+ T细胞为主（P<0.01），ICOS、CD28表达升高（P<0.05或P<0.01），肿瘤反应性T细胞比例升高（P<0.01）。结论：CRC的TDLN中T细胞处于免疫激活状态，同时高表达免疫抑制标志物；体外培养可以增加TDLN-T细胞活化水平，并提高抗肿瘤T细胞比例。
- Full text:20210902.pdf
