Ameliorative effect of psoralen on acute liver injury induced by carbon tetrachloride in mice
10.11665/j.issn.1000-5048.20210512
- VernacularTitle:补骨脂素对四氯化碳致小鼠急性肝损伤的改善作用
- Author:
Yan ZHANG
1
;
Zhenzhou JIANG
;
Luyong ZHANG
Author Information
1. 中国药科大学药物科学研究院新药筛选中心
- Publication Type:Journal Article
- Keywords:
psoralen;
carbon tetrachloride;
acute liver injury;
cytochrome P450 2E1
- From:
Journal of China Pharmaceutical University
2021;52(5):596-602
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the ameliorative effect of psoralen (PSO) on carbon tetrachloride (CCl4)-induced acute liver injury in mice and its potential mechanism, female C57BL/6J mice aged 6-8 weeks were continuously administrated with psoralen or positive control drug diallyl sulfide (DAS) intragastrically for 4 days.On day 4, except that the control group were treated with vehicle control, other groups were all given carbon tetrachloride intraperitoneally to establish a carbon tetrachloride acute liver injury model.Serum biochemical indicators alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels were detected; liver pathological changes were observed by HE staining; cytochrome P450 2E1 (CYP2E1) protein levels were detected by Western blot; the protein level of CYP2E1 were detected by immunohistochemistry (IHC) staining; and the gene levels of CYP2E1, tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were detected by RT-PCR.Compared with the model group, psoralen could improve the inflammatory cell infiltration and hepatocyte necrosis caused by carbon tetrachloride, significantly reducing the serum ALT and AST levels, down-regulating the inflammatory factors TNF-α and IL-6 levels, and inhibiting CYP2E1 protein expression.The results show that psoralen can ameliorate the acute liver injury induced by carbon tetrachloride in mice, with the possible mechanism inhibiting the protein expression of CYP2E1.
