Anti-asthmatic effect of agarwood alcohol extract in mice and its mechanism.
10.19540/j.cnki.cjcmm.20210520.704
- Author:
Can-Hong WANG
1
;
De-Qian PENG
2
;
Yang-Yang LIU
1
;
Yu-Lan WU
3
;
Peng GUO
4
;
Jian-He WEI
4
Author Information
1. Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine & Key Laboratory of State Administration of Traditional Chinese Medicine for Agarwood Sustainable Utilization, Hainan Branch Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College Haikou 570311, China.
2. School of Pharmacy, Hainan Medical University Haikou 571199, China.
3. Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine & Key Laboratory of State Administration of Traditional Chinese Medicine for Agarwood Sustainable Utilization, Hainan Branch Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College Haikou 570311, China School of Pharmacy, Hainan Medical University Haikou 571199, China.
4. Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine & Key Laboratory of State Administration of Traditional Chinese Medicine for Agarwood Sustainable Utilization, Hainan Branch Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College Haikou 570311, China Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, National Engineering Laboratory for Breeding of Endangered Medicinal Materials, Institute of Medicinal Plant Development, Chinese Academy of Medical Science & Peking Union Medical College Beijing 100193, China.
- Publication Type:Journal Article
- Keywords:
agarwood;
anti-apoptosis;
anti-asthmatic effect;
anti-inflammation
- MeSH:
Animals;
Anti-Asthmatic Agents;
Asthma/drug therapy*;
Bronchoalveolar Lavage Fluid;
Disease Models, Animal;
Lung;
Mice;
Mice, Inbred BALB C;
NF-kappa B;
Ovalbumin;
Plant Extracts/therapeutic use*
- From:
China Journal of Chinese Materia Medica
2021;46(16):4214-4221
- CountryChina
- Language:Chinese
-
Abstract:
As recorded, agarwood has the function of improving qi reception and relieving asthma, but the underlying mechanism is unclear and rarely reported. Therefore, this study explored the anti-asthmatic effect of the alcohol extract of agarwood produced by the whole-tree agarwood-inducing technique(Agar-Wit) in the asthma mouse model induced by intraperitoneal injection of ovalbumin(OVA) + Al(OH)_3 combined with intranasal administration of OVA and the mechanism, and compared the anti-asthmatic effects of agarwood induced with different methods. Firstly, the anti-inflammatory and anti-asthmatic effects of Agar-Wit agarwood in mice were evaluated based on the asthma frequency, lung tissue injury, and peripheral inflammatory white blood cell(WBC) count and eosinophil count. Then, the levels of interleukin-1β(IL-1β), IL-17, and IL-10 in serum of mice were detected by enzyme-linked immunoassay(ELISA) and the expression of inflammation-and apoptosis-related genes in tissues was measured by reverse transcription polyme-rase chain reaction(RT-PCR) so as to preliminarily explore the anti-asthmatic mechanism. RESULTS:: showed that the alcohol extract of Agar-Wit agarwood significantly reduced asthma frequency, relieved pathological injury, improved peripheral WBC count and eosinophil count, decreased the levels of inflammatory cytokines IL-1β and IL-17, elevated the level of anti-inflammatory cytokine IL-10, and down-regulated the mRNA expression of IL-1 R, tumor necrosis factor receptor R(TNFR), nuclear transcription factor-kappa B(NF-κB), Bax, and caspase 3, but had no significant influence on the expression of high-mobility group box 1(HMGB1) protein, caspase 8, and Bcl-2. The effect of Agar-Wit agarwood alcohol extract was better than that of wild agarwood alcohol extract and alcohol extract of agarwood induced with the burning-chisel-drilling method at the same dose. In conclusion, Agar-Wit agarwood can significantly alleviate inflammation and asthma, which is related to its anti-inflammation and anti-apoptosis activity.
