Determination of β-nicotinamide mononucleotide and nicotinamide adenine dinucleotide in Dendrobium officinale and congeneric species by UPLC-MS/MS.
10.19540/j.cnki.cjcmm.20210507.303
- Author:
Xiao-Qian LIU
1
;
Hong YANG
2
;
Jing-Yuan ZHAO
1
;
Chen-Xiao-Ning MENG
1
;
Chun LI
1
;
Dong ZHANG
1
;
Liang-Mian CHEN
1
;
Yu YAN
1
;
Zhong-Yuan GUO
1
;
Zhi-Min WANG
1
;
Hong YI
1
Author Information
1. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
2. Capital Medical University Beijing 100069, China.
- Publication Type:Journal Article
- Keywords:
Dendrobium;
Dendrobium officinale;
NAD~+;
NMN;
UPLC-MS/MS
- MeSH:
Chromatography, High Pressure Liquid;
Chromatography, Liquid;
Dendrobium;
NAD;
Nicotinamide Mononucleotide;
Tandem Mass Spectrometry
- From:
China Journal of Chinese Materia Medica
2021;46(16):4034-4039
- CountryChina
- Language:Chinese
-
Abstract:
As anti-aging ingredients, β-nicotinamide mononucleotide(NMN) and nicotinamide adenine dinucleotide(NAD~+) have attracted worldwide attention in recent years. After oral administration, NMN can be converted into NAD~+ in vivo and the latter is the actual ingredient which exerts anti-aging effect. In order to explore the "rejuvenating and anti-aging" effect of Dendrobium officinale, which was firstly recorded in Shennong's Herbal Classic of Materia Medica, this study established the quantitative method of UPLC-MS/MS for simultaneous determination of NMN and NAD~+ in D. officinale and the congeneric species for the first time, and 34 batches of samples were detected. UPLC conditions are as follows: ACQUITY UPLC HSS T3 column(2.1 mm × 100 mm, 1.8 μm), gradient elution with acetonitrile-0.1% formic acid in water at the flow rate of 0.3 mL·min~(-1), and column temperature of 40 ℃. MS conditions were scanned electrospray ionization source and multiple reaction monitoring mode. The method was verified by systematic methodology. The mean recoveries of NMN and NAD~+ were 77.58% and 80.70%, respectively, with RSD of 3.6% and 4.3%, separately. All results showed that the content of NMN was higher in D. officinale than in the other congeneric species. Particularly, the content in fresh D. officinale stems was as high as 0.931 9 μg·g~(-1). NAD~+ was only found in D. officinale and the content was three times higher than that of NMN. This may be the reason that D. officinale topped the "nine famous anti-aging herbs". In addition, processing method influences the content of NMN and NAD~+ in Dendrobium. Specifically, the content of NMN and NAD~+ was in the order of fresh Dendrobium stems > dried Dendrobium stem segments > spiral or spring-like dried Dendrobium stems.
