Mechanism of Xinfeng Capsules improving rheumatoid arthritis based on CD19~+B cells regulating FAK/CAPN/PI3K pathway.
10.19540/j.cnki.cjcmm.20201120.501
- Author:
Lei WAN
1
;
Jian LIU
1
;
Chuan-Bing HUANG
1
;
Lei ZHAO
2
;
Xi CHEN
1
;
Hai-Xia FAN
1
;
Yao GE
1
;
Tian-Yang LIU
1
;
Lei LIU
1
;
Zheng LI
3
;
Zi-Heng ZHU
2
;
Xi-Meng MA
2
;
Shu LI
2
Author Information
1. Department of Rheumatology,the First Affiliated Hospital of Anhui University of Chinese Medicine Hefei 230031,China.
2. Graduate School of Anhui University of Chinese Medicine Hefei 230031,China.
3. Department of Cancer Biology,City of Hope National Medical Center and Beckman Research Institute California 91010,USA.
- Publication Type:Journal Article
- Keywords:
B lymphocytes;
Xinfeng Capsules;
calpain;
focal adhesion kinase;
phosphoinositide-3 kinase;
rheumatoid arthritis
- MeSH:
Arthritis, Rheumatoid/drug therapy*;
B-Lymphocytes;
Capsules;
Drugs, Chinese Herbal;
Humans;
Phosphatidylinositol 3-Kinases;
Vascular Endothelial Growth Factor A
- From:
China Journal of Chinese Materia Medica
2021;46(14):3705-3711
- CountryChina
- Language:Chinese
-
Abstract:
To observe the effect of Xinfeng Capsules on rheumatoid arthritis (RA) B lymphocytes,inflammatory mediators,FAK/CAPN/PI3K pathway,in order to explore the mechanism of Xinfeng Capsules in improving clinical symptoms of RA.Joint and systemic symptoms of RA patients were observed,and laboratory indicators[hemoglobin (HGB),platelet count (PLT),erythrocyte sedimentation (ESR),immunoglobulin (Ig) G,Ig A,Ig M,rheumatoid factor (RF),anti-cyclic citrulline antibody (CCP-AB),C-reactive protein (CRP)]were detected.ELISA was used to detect serum interleukin (IL)-1β，IL-10,IL-33,chemokine 5 (CCL5),and vascular endothelial growth factor (VEGF).CD3~-CD19~+B cells were measured by flow cytometry.Western blot was used to detect FAK,p-FAK,CAPN,PI3K protein.The results showed that Xinfeng Capsules could significantly alleviate RA joint and systemic symptoms and improve clinical efficacy.And Xinfeng Capsules could increase HGB,decrease PLT,CCP-AB,CRP,ESR index,upregulate IL-10 expression,and down-regulate IL-1β，IL-33,CCL5,VEGF,CD3~-CD19~+B cells,FAK,p-FAK,CAPN,PI3K expressions (P<0.01).Based on the above results,Xinfeng Capsules may reduce the expression of CD3~-CD19~+,regulate the balance of inflammatory cytokines and chemokines,inhibit abnormal activation of FAK/CAPN/PI3K pathway,and improve clinical symptoms of RA.
