Analysis of volatile oil components of different species of Curcumae Rhizoma based on GC-MS and chemometrics.
10.19540/j.cnki.cjcmm.20210304.202
- Author:
Zhen-Wei LAN
1
;
Lyu-Hong WANG
1
;
Qi-Ting LI
1
;
Shu-Mei WANG
1
;
Jiang MENG
1
Author Information
1. Engineering Technology Research Center for Chinese Materia Medica Quality of Universities in Guangdong Province,Key Laboratory of Digital Quality Evaluation of Chinese Materia Medica,State Administration of Traditional Chinese Medicine ( TCM) ,School of TCM,Guangdong Pharmaceutical University Guangzhou 510006,China.
- Publication Type:Journal Article
- Keywords:
Curcumae Rhizoma;
GC-MS;
characteristic component;
chemometrics;
volatile oil
- MeSH:
Curcuma;
Gas Chromatography-Mass Spectrometry;
Oils, Volatile;
Principal Component Analysis;
Rhizome
- From:
China Journal of Chinese Materia Medica
2021;46(14):3614-3624
- CountryChina
- Language:Chinese
-
Abstract:
The volatile oil of Curcumae Rhizoma has many active components,which are the key to the quality of Curcumae Rhizoma. Exploring the difference between volatile oil of different kinds of Curcumae Rhizoma facilitates the quality control and rational application of resources. In this study,GC-MS was applied to realize online qualitative and semi-quantitative analysis of the chemical composition spectrum of volatile oil from Curcuma wenyujin( CW),C. phaeocaulis( CP),and C. kwangsiensis( CK). Forty components were identified and their fingerprints were compared and evaluated. Hierarchical cluster analysis( HCA),principal component analysis( PCA),and orthogonal partial least squares discrimination analysis( OPLS-DA) were adopted to analyze the overall and outlier data. The results showed that the whole data could be divided into three kinds according to each analysis mode,and the volatile components of Curcumae Rhizoma vary greatly among species. PCA explored the difference between outliers and the mean value of the group and found that some volatile oils from CW may be greatly affected by the origin. By OPLS-DA,the samples from Zhejiang were able to gather,but those from Guizhou remained isolated,indicating the influence of growing environment on Curcumae Rhizoma metabolites. Based on VIP results combined with the heat map,characteristic volatile oil components of Curcumae Rhizoma from different varieties were screened out: curdione and linalool for CW; 2-undecanone for CP; humulene,γ-selinene,and zederone for CK. The GCMS method established in this study describes Curcumae Rhizoma samples comprehensively and accurately,and the characteristic components screened based on chemometrics can be used to distinguish Curcumae Rhizoma from different varieties and give them unique pharmacodynamic significance,which is fast,convenient,stable,and reliable and supports the rational application of Curcu-mae Rhizoma resources. It is found that the region of origin has great influence on CW,which is worthy of further study.
