Evaluation of Multidrug Resistant Loop-mediated Isothermal Amplification Assay for Detecting the Drug Resistance of
- Author:
Chun Fa LIU
1
;
Yi Meng SONG
2
;
Ping HE
3
;
Dong Xin LIU
4
;
Wen Cong HE
3
;
Yan Ming LI
2
;
Yan Lin ZHAO
1
Author Information
- Publication Type:Journal Article
- Keywords: Diagnostic method; MDR-LAMP; Mycobacterium tuberculosis
- MeSH: Antitubercular Agents; Bacterial Proteins/genetics*; Catalase/genetics*; DNA, Bacterial/analysis*; DNA-Directed RNA Polymerases/genetics*; Drug Resistance, Multiple, Bacterial/genetics*; Isoniazid; Molecular Diagnostic Techniques/methods*; Mutation; Mycobacterium tuberculosis/isolation & purification*; Nucleic Acid Amplification Techniques/methods*; Oxidoreductases/genetics*; Phenotype; Rifampin; Whole Genome Sequencing
- From: Biomedical and Environmental Sciences 2021;34(8):616-622
- CountryChina
- Language:English
-
Abstract:
Objective:To evaluate multidrug resistant loop-mediated isothermal amplification (MDR-LAMP) assay for the early diagnosis of multidrug-resistant tuberculosis and to compare the mutation patterns associated with the
Methods:MDR-LAMP assay was evaluated using 100
Results:The sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of MDR-LAMP were 85.5%, 93.6%, 96.7%, and 74.4% for the detection of resistance to isoniazid and rifampicin, respectively, and 80.5%, 92.3%, 98.6%, and 41.4% for the detection of
Conclusion:MDR-LAMP is a rapid and accessible assay for the laboratory identification of rifampicin and isoniazid resistance of
