Degrading enzyme modified molecularly imprinted xerogel for selective adsorption and degradation of dopamine
10.16438/j.0513-4870.2020-1935
- VernacularTitle:经降解酶修饰的分子印迹干凝胶用于多巴胺选择性吸附和降解
- Author:
Xiao-chang LI
1
;
Tao-xi CHEN
2
;
Qing-chen LI
1
;
Yang-jia LIU
2
;
Tian-tian YE
2
;
Shu-jun WANG
2
Author Information
1. School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China
2. School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China
- Publication Type:Research Article
- Keywords:
molecularly imprinted polymer;
selective;
adsorption;
opamine;
monoamine oxidase;
sol-gel process
- From:
Acta Pharmaceutica Sinica
2021;56(5):1470-1477
- CountryChina
- Language:Chinese
-
Abstract:
In recent years, the overexpression dopamine (DA) due to the use of addictive drugs has caused concern and urgently needs to be addressed. The method used in our study is known as biomimetic sol-gel synthesis. We undertook the experiment to develop molecularly imprinted xerogel polymers (MIXPs) through template molecules dopamine polymerized with polyethyleneimine (PEI), then self-assembled and crosslinked with tetramethoxysilane (TMOS) in the form of non-covalent hydrogen bonds by using biomimetic sol-gel process, and then eluted template DA will leave a blotting site. Monoamine oxidase immobilized MIXPs (MAO-MIXPs) was obtained by coating monoamine oxidase onto MIXPs. The synthesis optimization of MAO-MIXPs was finally set as the ratio of DA template, PEI and MAO coating (DA 40 mg, PEI 0.6 mL, MAO 2.5 mg·g-1) to achieve highly selective adsorption toward DA in artificial cerebrospinal fluid based on the adsorption performance and degradation performance. The micromorphologies and physical-chemical properties of MAO-MIXPs were characterized by scanning electron microscopy, differential scanning calorimeter and Fourier transform infrared spectroscopy, and then amount of adsorption was calculated with adsorption equation. Simultaneously, the Brunner-Emmet-Teller (BET) and Langmuir model were simulated. It was found that the adsorption behavior tended to be monolayer adsorption. This new molecularly imprinted polymer demonstrated potential dopamine expression regulation for highly selective recognition, adsorption and degradation of dopamine.
