miR-206 inhibits the proliferation and invasion of estrogen-induced ER-α36-positive gastric cancer BGC-823 cells by targeting CDK14
DOI：10.3872/j.issn.1007-385x.2021.07.006
- VernacularTitle:miR-206通过靶向CDK14抑制雌激素诱导的ER-α36阳性胃癌BGC-823细胞的增殖和侵袭
- Author:
DU Wenkai
1
;
ZHANG Yiyin
2
;
DU Wei
3
Author Information
1. Department of Oncology, Huaibei People's Hospital of Anhui Province, Huaibei 235000, Anhui, China
2. Department of Oncology, Gaoxin Branch of the First Affiliated Hospital of Anhui Medical University, Hefei 230022, Anhui, China
3. Fifth Department of Internal Medicine, Suixi County Hospital of Traditional Chinese Medicine, Huaibei 235000, Anhui, China
- Publication Type:Journal Article
- Keywords:
胃癌；BGC-823细胞；miR-206；CDK14；雌二醇；增殖；侵袭
- From:
Chinese Journal of Cancer Biotherapy
2021;28(7):689-695
- CountryChina
- Language:Chinese
-
Abstract:
[摘 要] 目的：探讨miR-206对雌激素诱导的ER-α36阳性胃癌（gastric cancer, GC）细胞BGC-823增殖和侵袭的影响及其相关机制。方法: 用不同浓度（1、10和100 pmol/L）的雌二醇（estradiol，E2）刺激ER-α36阳性BGC-823细胞后，用qPCR法检测miR-206表达水平，MTT法和Transwell实验分别检测细胞的增殖和侵袭能力，WB法检测细胞中CDK14的表达。将miR-206 mimic、miR-NC、pcDNA-CDK14、pcDNA-vector等转染ER-α36阳性BGC-823细胞，并给予100 pmol/L的E2处理后，用MTT法和Transwell小室法分别检测细胞的增殖和侵袭能力，WB法检测细胞中CDK14的表达。用双荧光素酶报告基因实验验证miR-206与CDK14之间的靶向关系。结果: E2能显著降低ER-α36阳性BGC-823细胞中miR-206表达水平（P<0.05或P<0.01）、增强细胞的增殖和侵袭能力（P<0.05或P<0.01）、上调细胞中CDK14的表达水平（P<0.01）。过表达miR-206能显著降低E2诱导的ER-α36阳性BGC-823细胞的增殖和侵袭能力（均P<0.01）。miR-206通过直接结合CDK14 mRNA的3'-UTR发挥抑制作用，从而负向调节CDK14的表达，进而抑制ER-α阳性BGC-823细胞的增殖和侵袭能力（均P<0.01）。结论: miR-206通过靶向CDK14从而抑制雌激素诱导的ER-α36阳性GC细胞的增殖和侵袭。
- Full text:20210706.pdf
