Expression of miR-203a-3p in pancreatic cancer tissues and cells and its effect on proliferation, migration and invasion of BxPC-3 cells
DOI：10.3872/j.issn.1007-385x.2021.07.005
- VernacularTitle:miR-203a-3p在胰腺癌组织与细胞中的表达及其对BxPC-3细胞增殖、迁移和侵袭的影响
- Author:
LU Hongjian
1
;
ZHANG Ronghua
1
;
HUO Xiaoju
2
;
HAN Xiangyang
1
;
WANG Yuan
1
;
YANG Chen
3
;
MA Ruixue
3
;
ZHANG Guangling
3
Author Information
1. a. Hebei Provincial Key Laboratory for Chronic Diseases, School of Basic Medical Sciences
2. b. Affiliated Hospital, School of Clinical Medicine, North China University of Science and Technology, Tangshan 063000, Hebei, China
3. c. Hebei Provincial Key Laboratory of Medical-Industrial Integration Precision Medicine, School of Clinical Medicine, North China University of Science and Technology, Tangshan 063000, Hebei, China
- Publication Type:Journal Article
- Keywords:
miR-203a-3p；胰腺癌；BxPC-3细胞；增殖；迁移；侵袭
- From:
Chinese Journal of Cancer Biotherapy
2021;28(7):680-688
- CountryChina
- Language:Chinese
-
Abstract:
[摘 要] 目的：探讨miR-203a-3p对胰腺癌BxPC-3细胞增殖、迁移和侵袭能力的影响。方法：运用癌症基因组图谱（TCGA）数据库筛选胰腺癌组织和癌旁组织中差异表达的miRNA，分析miRNA高表达与低表达时胰腺癌患者的生存率和临床分期；利用TarBase数据库分析miRNA与癌症相关的GO功能与KEGG通路，利用DIANA Tools、miRDB和TargetScan网站预测miR-203a-3p的靶基因。将miR-203a-3p mimic及NC mimic、miR-203a-3p inhibitor及NC inhibitor转染至BxPC-3细胞，用qPCR法检测胰腺癌细胞和胰腺正常上皮细胞HPNE中miR-203a-3p、miR-192-5p和miR-451a表达水平，以CCK-8法、Transwell小室法和克隆形成实验分别检测BxPC-3细胞的增殖、迁移、侵袭和集落形成能力。结果：通过TCGA数据库筛选出18个胰腺癌组织中差异表达的miRNA，其中miR-203a-3p、miR-192-5p、miR-451a具有物种保守性，且其与胰腺癌临床癌症分期、细胞周期和患者生存率相关（均P<0.05）；生物信息学网站预测显示miR-203a-3p的候选靶基因是PPM1A，PPM1A与多基因存在相互作用。miR-203a-3p、miR-192-5p和miR-451a在BxPC-3和Aspc-1细胞中均高表达（均P<0.01）。miR-203a-3p mimic组BxPC-3细胞中miR-203a-3p表达水平以及细胞增殖、迁移和侵袭能力均显著提高（均P<0.01）；miR-203a-3p inhibitor组细胞中miR-203a-3p表达水平以及细胞增殖、迁移和侵袭能力均显著降低（均P<0.01）。结论：miR-203a-3p在胰腺癌组织及细胞中均高表达，其表达与患者生存和临床分期相关，可调控BxPC-3细胞的增殖、迁移和侵袭能力。
- Full text:20210705.pdf
