Effects of temperature, pH, and H2O2 on the growth and biofilm formation of attenuated Salmonella VNP20009
10.16438/j.0513-4870.2021-0175
- VernacularTitle:温度、pH值和H2O2对减毒沙门氏菌VNP20009的生长及生物膜形成的影响
- Author:
Jing LI
1
;
Fei-fei BAO
1
;
Jia-huang LI
2
;
Zi-chun HUA
2
Author Information
1. State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210023, China
2. State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210023, China; College of Biopharmaceuticals, China Pharmaceutical University, Nanjing 211198, China; High-Tech Research Institute of Nanjing University at Changzhou and Jiangsu Target Pharma Laboratories Inc., Changzhou 213164, China
- Publication Type:Research Article
- Keywords:
italic>Salmonella;
VNP20009;
growth;
biofilm;
microbiological phenomena
- From:
Acta Pharmaceutica Sinica
2021;56(6):1606-1611
- CountryChina
- Language:Chinese
-
Abstract:
Attenuated Salmonella VNP20009 specifically colonizes and proliferates within tumor tissues and inhibits tumor growth. It has been used as drug delivery vehicle or in combination with other therapies (such as chemotherapy), which shows a good application potential in tumor therapy. In this paper, study was conducted to determine the physiological changes of growth curve and formation of bacterial biofilm of VNP20009 under various environmental stresses, such as temperature, pH, and H2O2. The results showed that VNP20009 could grow normally under the conditions of 42 ℃, pH 6.5, and 1 mmol·L-1 H2O2. Furthermore, the weak acid environment was beneficial to the biofilm formation of VNP20009. This study provides a basis for in-depth study of the survival mechanism and application of attenuated Salmonella.
