Molecular analysis of the first dengue fever case in Dapeng New District of Shenzhen City
10.19428/j.cnki.sjpm.2021.20023
- VernacularTitle:深圳市大鹏新区首发登革热病例的分子病毒学分析
- Author:
Xian-qiang LIAN
1
;
Yue YANG
1
;
Wen-xiang WANG
1
;
Jian-bo ZHANG
1
Author Information
1. Dapeng New District Center for Public Health Management and Service， Shenzhen， Guangdong 518116， China
- Publication Type:Research Article
- Keywords:
dengue fever;
dengue virus type 1;
E gene;
phylogenetic tree
- From:
Shanghai Journal of Preventive Medicine
2021;33(6):467-470
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the first suspected dengue fever case in Dapeng New District by characterizing the virus and exploring its origin. Methods:Dengue virus nucleic acid in blood sample was identified by real-time fluorescent RT-PCR. The E gene of dengue virus was amplified and sequenced. Homology and phylogenetic tree of this dengue virus were compared with the isolates from other regions. Results:Nucleic acid of dengue virus type 1 was detected in the serum sample from the suspected dengue fever patient. Phylogenetic tree showed that the homology based on nucleotide sequence of E gene of DP-DGR19001 was very similar with the isolates of MG894965/TW/CHN/2016 （99.87%）， LC428079/VNM/2017（99.80%）， MG894867/TW/CHN/2015 （99.60%） and MN512242/GX/CHN/2014 （99.46%）. The deduced amino acid sequence of 5 isolates was 100% identical. The patient had traveled to high-incidence country Cambodia in 14 days before infection. Conclusions:The first suspected dengue fever case in Dapeng New District is caused by dengue virus type 1. The case is an imported dengue fever .
