Effect of Modified Xijiao Dihuang Decoction () on Intestinal Flora and Th17/Treg in Rats with Radiation Enteritis.
10.1007/s11655-020-3261-x
- Author:
Yu-Guo WANG
1
;
Yue GAO
2
;
Jian FENG
1
;
Yong-Qi DOU
3
Author Information
1. Medical School of Chinese People's Liberation Army, The General Hospital of People's Liberation Army, Beijing, 100853, China.
2. Beijing Institute of Radiation Medicine, Beijing, 100850, China.
3. Medical School of Chinese People's Liberation Army, The General Hospital of People's Liberation Army, Beijing, 100853, China. dyqi_301@yeah.net.
- Publication Type:Journal Article
- Keywords:
Chinese medicine;
Modified Xijiao Dihuang Decoction;
Th17/Treg;
intestinal flora;
radiation enteritis
- From:
Chinese journal of integrative medicine
2021;27(3):198-205
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:To observe the effect of Modified Xijiao Dihuang Decoction (, MXDD) on rats with radiation enteritis, and explore its action mechanism.
METHODS:Thirty female Sprague Dawley rats were divided into the control, model, dexamethasone (DXM), golden bifid (GB) and MXDD groups using random number table, 6 rats in each group. Except the control group, the other rats were developed into radiation enteritis model by exposing to a single
RESULTS:On day 1 to 3 after radiation, compared with the control group, the body weight in model group was decreased (P<0.05 or P<0.01). Compared with the model group, MXDD could alleviate weight loss and diarrhea caused by irradiation. At the phylum level, MXDD cause a significant increase in Firmicutes, and a decrease in Proteobacteria (P<0.05 or P<0.01). At the genus level, MXDD reduced the proportion of Escherichia Shigella (P<0.01). In addition, IL-17 and FoxP3 mRNA and protein expression levels were down-regulated and ROR-γt was up-regulated by MXDD treatment (P<0.05). Besides, Firmicutes and Lactobacillus were positively correlated with FoxP3 (r=0.73, 0.79, respectively; P<0.01), negatively correlated with IL-17 (r=0.66, 0.64, respectively; P<0.01 or P<0.05) and ROR-γt (r0.73, 0.81, respectively; P<0.01). Proteobacteria and Escherichia Shigella both had positive correlation with IL-17 (r 0.77, 0.57, respectively; P<0.01 or P<0.05 ) and ROR-γt (r=0.94, 0.79, respectively; P<0.01) and negative correlation with FoxP3 (r0.74, 0.65; P<0.01).
CONCLUSION:MXDD could improve the survival status of irradiated rats by regulating the richness, diversity and composition of intestinal flora, and restoring the balance of Th17/Treg.
