Therapeutic Effect of Spleen Low Molecular Weight Extracts on Leukopenia Caused by Epirubicin in Mice and Its Mechanism.
10.19746/j.cnki.issn.1009-2137.2021.03.050
- Author:
Yi-Ting LIN
1
;
Xin-Yue ZHENG
2
;
Yi-Fan YAO
1
;
Yu-Ying ZHANG
1
;
Ting-Ting HUANG
1
;
Yun-Lan ZHU
1
;
Jun PEI
1
;
Jin WANG
2
;
Ming CHU
1
;
Yue-Dan WANG
3
Author Information
1. Department of Immunology, School of Basic Medicine, Peking University, Beijing 100191, China.
2. Beijing No.1 Biochemical Pharmaceutical Co., Ltd., Beijing 101401, China.
3. Department of Immunology, School of Basic Medicine, Peking University, Beijing 100191, China,E-mail：wangyuedan@bjmu.edu.cn.
- Publication Type:Journal Article
- MeSH:
Animals;
Epirubicin;
Granulocyte Colony-Stimulating Factor;
Leukocyte Count;
Leukopenia/drug therapy*;
Mice;
Molecular Weight;
Plant Extracts;
Recombinant Proteins;
Spleen
- From:
Journal of Experimental Hematology
2021;29(3):969-974
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the therapeutic effect of spleen low molecular weight extracts on epileptics hydrochloride-induced leukopenia in mice and explore its mechanism.
METHODS:The model of leukopenia in mice was established by the injection of epirubicin hydrochloride (10 mg/kg). After the injection of chemotherapeutic drugs, leukocytopenia mice were treated with different doses of spleen low molecular weight extract, Ganoderma oral solution and recombinant granulocyte colony stimulating factor (rhG-CSF). The general survival status indicators such as body weight, coat color and athletic ability of mice in each group were recorded; the tail vein blood of mice in each group was collected and the white blood cell count in them was calculated; bone marrow of mice was taken and bone marrow smears were observed.
RESULTS:In the model group, the weight of the mice gradually decreased in the later period, their coat became dark and rough, and the ability to exercise decreased, while the mice in the treatment groups showed different degrees of improvement in their survival status except for the mice treated by rhG-CSF. There was no significant fluctuation in the white blood cell count of the blank control mice. After injection of epirubicin, the white blood cell count of peripheral blood in the model mice and treated mice were decreased. The white blood cell count was lower in the mice treated with high-dose low molecular weight extract and rhG-CSF than that in other experimental groups. Bone marrow smear showed that the proportion of bone marrow nucleated cells in the mice treated with the low molecular weight extract of the spleen was significantly higher than that of model mice (P<0.05).
CONCLUSION:The low molecular weight spleen extracts can significantly improve the hematopoietic state of mouse bone marrow, promote the proliferation of inhibited bone marrow cells, and thus has the effect of treating leukopenia in mice.
