Study on protective effect of total flavonoids from Rosa multiflora on HUVEC induced by ox-LDL.
10.19540/j.cnki.cjcmm.20201201.402
- Author:
Dan YANG
1
;
Jun WANG
1
;
Kai-Yan LI
1
Author Information
1. Henan Provincial Academy of Traditional Chinese Medicine Zhengzhou 450004,China.
- Publication Type:Journal Article
- Keywords:
adhesion molecule;
human umbilical vein endothelial cells;
inflammatory factors;
oxidized low density lipoprotein;
total flavonoids from Rosa multiflora
- MeSH:
Animals;
Cells, Cultured;
Endothelial Cells;
Endothelium, Vascular;
Flavonoids/pharmacology*;
Intercellular Adhesion Molecule-1/genetics*;
Lipoproteins, LDL;
Male;
Rats;
Rats, Sprague-Dawley;
Rosa
- From:
China Journal of Chinese Materia Medica
2021;46(5):1211-1216
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this paper was to study the protective effect of total flavonoids from Rosa multiflora(TF-RM) on the injury of HUVEC induced by oxidized low density lipoprotein(ox-LDL). SPF male SD rats were randomly divided into blank group, simvastatin group(1.8 mg·kg~(-1)·d~(-1)) and TF-RM group(2.5 g·kg~(-1)·d~(-1)), with 10 rats in each group. They were intragastrically administered with drugs for 7 days, and then blood was collected from the abdominal aorta to prepare drug-containing serum. The HUVEC injury model was established through ox-LDL induction, and added with 15% simvastatin, 5% TF-RM, 10% TF-RM, 15% TF-RM drug-containing serum and blank serum, respectively. Reactive oxygen species(ROS) was determined by flow cytometry. Nitric oxide(NO) content was determined by nitrate reductase method. The contents of ET-1, P-selectin, E-selectin, ICAM-1, VCAM-1, IL-1β, IL-6 and TNF-α were determined by ELISA. The expression of Lox-1 protein was determined by Western blot. Compared with the blank group, ROS level in HUVEC and the contents of ET-1, P-selectin, E-selectin, ICAM-1, VCAM-1 and IL-1β in HUVEC were significantly increased(P<0.05), NO decreased significantly(P<0.01),Lox-1 protein expression increased significantly(P<0.05), and TNF-α and IL-6 showed an increasing trend. Compared with the model group, TF-RM significantly reduced ROS level in HUVEC and ET-1, P-selectin, E-selectin, ICAM-1, TNF-α, IL-1β content in supernatant(P<0.05), significantly increased NO content(P<0.01), and inhibited Lox-1 protein expression(P<0.05). VCAM-1, IL-6 contents showed a decreasing trend. Serum containing TF-RM acts on lectin-like oxidized low-density lipoprotein receptors, and exerts a protective effect on vascular endothelial cells by reducing cell oxidative damage, regulating vasoactive substances, and reducing adhesion molecules and inflammatory cascades.
