Effect of swertiamarin, gentiopicrin and sweroside on cell apoptosis and expression of Bcl-2 in rheumatoid arthritis fibroblast-like synoviocytes.
10.19540/j.cnki.cjcmm.20200927.401
- Author:
Chen WU
1
;
Yun WEI
1
;
Shan GE
1
;
Hui-Qin GAO
1
Author Information
1. School of Pharmacy, Gansu University of Chinese Medicine Lanzhou 730000, China.
- Publication Type:Journal Article
- Keywords:
Bcl-2;
fibroblast-like synoviocytes;
gentiopicrin;
molecular docking;
rheumatoid arthritis;
sweroside;
swertiamarin
- MeSH:
Apoptosis;
Arthritis, Rheumatoid/genetics*;
Cell Proliferation;
Cells, Cultured;
Fibroblasts;
Humans;
Iridoid Glucosides;
Molecular Docking Simulation;
Proto-Oncogene Proteins c-bcl-2/genetics*;
Pyrones;
Synoviocytes
- From:
China Journal of Chinese Materia Medica
2021;46(2):406-411
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this paper was to discuss the effect of swertiamarin, gentiopicrin and sweroside on rheumatoid arthritis fibroblast-like synoviocytes(RA-FLSs) and B-cell lymphoma-2(Bcl-2) and their mechanisms. ZINC database and RCSB PDB database were retrieved for 3 D chemical structures of swertiamarin, gentiopicrin and sweroside and 3 D target protein structures. AutoDock Mgltools 1.5.6, AutoDockVina 1.1.2 and pyMOL 2.2.0 were applied for molecular docking to analyze the relationship between Bcl-2(1 GJH) target protein and important ingredients. The cell apoptosis of RA-FLSs was tested by Annexin V-FITC. The Bcl-2 protein expression of RA-FLSs treated with different ingredients was tested by Western blot. The Bcl-2 mRNA expression of RA-FLSs treated with different ingredients was tested by RT-PCR. Swertiamarin, gentiopicrin and sweroside were docked well with Bcl-2(1 GJH). The binding energy of swertiamarin was-6.9 kcal·mol~(-1), the binding energy of gentiopicrin was-6.7 kcal·mol~(-1) and the binding energy of sweroside was-6.4 kcal·mol~(-1). Compared with the blank group, the Bcl-2 protein expression of each group were reduced, while that of the gentiopicrin group was the highest(P<0.01). Compared with the blank group, the Bcl-2 mRNA expression of each groups were reduced. Gentiopicrin can reduce the Bcl-2 protein expression and the Bcl-2 mRNA expression, so as to promote the RA-FLSs apoptosis.
