Assessment of Benchmark Dose in BEAS-2B Cells by Evaluating the Cell Relative Viability with Particulates in Motorcycle Exhaust
- Author:
Tao YU
1
;
Xue Yan ZHANG
1
;
Shu Fei LI
2
;
Yu Mei ZHOU
1
;
Bin LI
1
;
Zhong Xu WANG
1
;
Yu Fei DAI
1
;
Sherleen Xue-Fu ADAMSON
3
;
Yu Xin ZHENG
4
;
Ping BIN
1
Author Information
- Publication Type:Journal Article
- Keywords: Air–liquid interface; Bronchial epithelial cells; Cell relative viability; Dose-dependent effect; Motorcycle exhaust particulates
- MeSH: Benchmarking/statistics & numerical data*; Bronchi/physiology*; Cell Line; Cell Survival/drug effects*; Epithelial Cells/physiology*; Humans; Motorcycles; Particulate Matter/adverse effects*; Vehicle Emissions/analysis*
- From: Biomedical and Environmental Sciences 2021;34(4):272-281
- CountryChina
- Language:English
-
Abstract:
Objective:This study aimed to use an air-liquid interface (ALI) exposure system to simulate the inhalation exposure of motorcycle exhaust particulates (MEPs) and then investigate the benchmark dose (BMD) of MEPs by evaluating cell relative viability (CRV) in lung epithelial BEAS-2B cells.
Methods:The MEPs dose was characterized by measuring the number concentration (NC), surface area concentration (SAC), and mass concentration (MC). BEAS-2B cells were exposed to MEPs at different concentrations
Results:Our results reveal that BMD of NC and SAC were estimated by the best-fitting Hill model, while MC was estimated by Polynomial model. The BMDL for CRV following ALI exposure to MEPs were as follows: 364.2#/cm
Conclusion:These results indicate that MEPs exposure
