Effect of moxibustion on PI3K/Akt/mTOR signaling pathway in foot-pad synovium in rats with rheumatoid arthritis.
10.13703/j.0255-2930.20200112-k0003
- Author:
Feng HAO
1
;
Li-Bin WU
2
;
Jun HU
3
;
Lei LIU
2
;
Zi-Jian WU
4
;
Rong-Lin CAI
4
;
Ling HU
4
;
Xiao-Cun YANG
1
;
Jie WANG
2
;
Qing YU
2
;
Lu HE
2
Author Information
1. College of Acupuncture-Moxibustion and Tuina, Nanjing University of Chinese Medicine, Nanjing 210023, Jiangsu Province, China.
2. College of Acupuncture-Moxibustion and Tuina, Anhui University of Chinese Medicine.
3. First Clinical Medical College of Nanjing University of Chinese Medicine.
4. Institute of Acupuncture-Moxibustion and Meridian, Anhui Academy of TCM, Hefei
- Publication Type:Journal Article
- Keywords:
PI3K/Akt/mTOR signaling pathway;
autophagy;
inflammatory reaction;
moxibustion;
rheumatoid arthritis
- MeSH:
Animals;
Arthritis, Rheumatoid/therapy*;
Moxibustion;
Phosphatidylinositol 3-Kinases;
Proto-Oncogene Proteins c-akt;
Rats;
Rats, Sprague-Dawley;
Signal Transduction;
Synovial Membrane;
TOR Serine-Threonine Kinases
- From:
Chinese Acupuncture & Moxibustion
2020;40(11):1211-1216
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To observe the effect of moxibustion on phosphatidylinositol 3-kinase/protein kinase B/mammalian target of rapamycin protein (PI3K/Akt/mTOR) signaling pathway in the foot-pad synovial tissue in rats with rheumatoid arthritis (RA), and to explore the mechanism of moxibustion for treating RA.
METHODS:Forty healthy SD rats were randomly divided into a control group, a model group, a moxibustion group, a cigarette-moxibustion group and a medication group, 8 rats in each group. The RA model was established with subcutaneous injection of complete Freund's adjuvant (CFA) in the left hind foot-pad under wind, cold and wet environment in the model group, the moxibustion group, the cigarette-moxibustion group and the medication group. The rats in the moxibustion group were treated with moxibustion at "Zusanli" (ST 36) for 20 min; the rats in the cigarette-moxibustion group were treated with moxibustion of ordinary cigarette at "Zusanli" (ST 36) for 20 min; the rats in the medication group were treated with tripterygium glycosides suspension (0.8 mg/100 g) by gavage. All the intervention was given once a day for 15 days. The left hind foot-pad volume was measured before and after modeling and after 15-day intervention. After 15-day intervention, the serum levels of IL-17 and IL-23 were detected by ELISA method, and the expression levels of PI3K, Akt and mTOR in synovial tissue of left hind foot-pad were detected by Western blot method.
RESULTS:The volume of left hind foot-pad, the serum levels of IL-23 and IL-17 and the expression of PI3K, Akt and mTOR in synovial tissue of left hind foot-pad in the model group were higher than those in the control group (
CONCLUSION:Moxibustion may play a therapeutic effect on RA by inhibiting the level of IL-23, IL-17 and the activity PI3K/Akt/mTOR, and regulating inflammatory response and autophagy.
