Application progress of time-resolved fluorescence in immunoassay
10.3969/j.issn.1006-2483.2021.03.026
- VernacularTitle:时间分辨荧光在免疫分析中的应用进展
- Author:
Yuan LI
1
;
Xiang MAO
2
;
Jun HU
1
;
Hai YANG
1
;
Huai WANG
2
Author Information
1. College of Life Science and Technology, National Engineering Research Center for Nanomedicine, Huazhong University of Science and Technology, Wuhan 430074,China
2. Wuhan Center for Disease Control and Prevention, Wuhan 430015, China
- Publication Type:Journal Article
- Keywords:
Time-resolved fluorescence;
Chelating agent;
Rare earth doped nanoparticles;
Immunodiagnosis
- From:
Journal of Public Health and Preventive Medicine
2021;32(3):111-116
- CountryChina
- Language:Chinese
-
Abstract:
Time resolved fluoroimmunoassay (TRFIA) is an immunoassay technology developed on the basis of the unique fluorescence properties of rare earth elements. TRFIA combines the advantages of radioimmunoassay, enzyme-linked immunoassay and common fluorescence immunoassay. It has high sensitivity, strong specificity, good stability, wider measurement range, long fluorescence life, simple operation and non-radiation, and shows a good prospect in the field of immunoassay. In this paper, several common TRFIA materials are discussed based on the latest research progress of time-resolved fluorescence in immunoassay. The application of TRFIA in immunodiagnosis, food detection, environmental monitoring and so on is elaborated, and its development direction and application are prospected.
