Effect of Ermiaosan on M1/M2 Polarization of Bone Marrow Derived-macrophages
10.13422/j.cnki.syfjx.20200937
- VernacularTitle:二妙散对大鼠骨髓来源的巨噬细胞M1/M2极化的影响
- Author:
Lian-hua HE
1
;
Qing-xia QIN
1
;
Han WANG
1
;
Wen-cheng HE
1
;
Qing-wen WANG
1
Author Information
1. Shenzhen Hospital， Peking University， Shenzhen 518036, China
- Publication Type:Research Article
- Keywords:
Ermiaosan;
rheumatoid arthritis;
bone marrow derived;
macrophages;
M1/M2 polarization
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(11):71-77
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effect of Ermiaosan（EMS） on the polarization of M1 by lipopolysaccharide（LPS）+interferon(IFN)-γ and M2 induced by interleukin(IL)-4+IL-13 in rat bone marrow-derived macrophages. Method:Macrophages from rat bone marrow were extracted in vitro, stimulated by macrophage colony stimulating factor(M-CSF), induced to macrophages (marked by F4/80), stimulated by LPS+IFN-γ and induced to polarize to M1，while stimulated by IL-4+IL-13 and induced to polarize to M2. After adding different concentrations of EMS (0.2，0.4，0.8 g·L-1), the phenotypes of M1 and M2 were detected by immunofluorescence, and the effect of EMS on M1(marked by CD68 and iNOS)/M2(marked by CD206 and Arginase) polarization of macrophages from rat bone marrow was detected. Result:Compared with control group, LPS + IFN-γ could increase the polarization of M1 (P<0.01)，while IL-4+IL-13 could increase the polarization of M2 (P<0.01); compared with LPS+IFN-γ/IL-4+IL-13 group, EMS (0.2，0.4，0.8 g·L-1) could inhibit the polarization of M1 induced by LPS+IFN -γ for 24 hours (P<0.05), but had no significant effect on polarization of M2 induced by IL-4+IL-13. Conclusion:EMS can inhibit M1 polarization induced by LPS+IFN - γ, but has no effect on M2 polarization induced by IL-4+IL-13.
