Rapid Identification and Analysis of Chemical Components in Heartwood of Dalbergia cochinchinensis by UPLC-Q-TOF-MS/MS
10.13422/j.cnki.syfjx.20200647
- VernacularTitle:基于UPLC-Q-TOF-MS/MS技术快速鉴定交趾黄檀心材中化学成分
- Author:
Xiao-wei MENG
1
;
Ding-ji CAI
1
;
Qing ZHU
1
;
Huan-gang ZHANG
1
;
Meng-fei WANG
1
;
Yang LIU
1
;
Fang-rui TANG
1
;
Feng SHAO
1
;
Lan-ying CHEN
1
;
Rong-hua LIU
1
Author Information
1. Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China
- Publication Type:Research Article
- Keywords:
Dalbergia;
heartwood of D. cochinchinensis;
ultra high performance liquid chromatography-quadrupole-time-of-flight high resolution mass spectrometry (UPLC-Q-TOF-MS/MS);
chemical constituents;
flavonoids;
isoflavones;
neoflavonoids
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(9):143-156
- CountryChina
- Language:Chinese
-
Abstract:
Objective::To rapidly identify and analysis the chemical constituents in the methanol extract of heartwood of Dalbergia cochinchinensis by ultra high performance liquid chromatography-quadrupole-time-of-flight high resolution mass spectrometry (UPLC-Q-TOF-MS/MS). Method::UPLC RRHD SB-C18 column (3.0 mm×100 mm, 1.8 μm) was used for chromatographic separation with acetonitrile-0.1% formic acid solution as the mobile phase for gradient elution (0-0.01 min, 5%B; 0.01-2 min, 5%-22%B; 2-28 min, 22%-35%B; 28-45 min, 35%-44%B; 45-55 min, 44%-100%B; 55-57 min, 100%B; 57-57.10 min, 100%~5%B) at a flow rate of 0.3 mL·min-1. The analytes were determined in negative ion mode with electrospray ionization (ESI) and data collection range of m/z 100-1 500. Result::A total of 101 chemical constituents were identified, including 22 flavonoids, 34 isoflavones, 15 neoflavonoids, 18 other flavonoids and 12 other components. Conclusion::UPLC-Q-TOF-MS/MS technique can quickly, accurately and comprehensively identify the chemical constituents in the heartwood of D. cochinchinensis. Isoflavones, flavonoids and neoflavonoids are the main chemical constituents in the heartwood of D. cochinchinensis, which is of great significance to reveal its internal material basis and provides experimental basis for this plant to be developed as a potential new resource of traditional Chinese medicine.
