Qualitative Analysis on Perilla frutescens Leaves and Stalks by UPLC-Q-Exactive-Orbitrap-MS
10.13422/j.cnki.syfjx.20201512
- VernacularTitle:基于UPLC-Q-Exactive-Orbitrap-MS的紫苏叶与紫苏梗化学成分分析
- Author:
Qian-li KANG
1
;
Zhuang-zhuang LI
1
;
Shan-shan FAN
1
;
Rong RONG
1
;
Hai-qiang JIANG
1
;
Xiao-lin JIANG
1
;
Ji-qiu ZHANG
1
;
Li-li GONG
1
Author Information
1. Experience Center， School of Pharmaceutical Science， Shandong University of Traditional Chinese Medicine， Ji'nan 250355， China
- Publication Type:Research Article
- Keywords:
Perilla frutescens leaves;
P. frutescens stalk;
liquid chromatography-mass spectrometry(LC-MS);
component identification;
triterpene acid
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(13):156-162
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method for qualitative analysis of components in Perilla frutescens leaves and stalks by liquid chromatography-mass spectrometry (LC-MS)，so as to explore the substance basis of pharmacodynamics differences between P.frutescens leaves and stalks.Method:P. frutescens leaves and stalks were extracted by 80% methanol-water ultrasound. The samples were analyzed by UPLC-Q-Exactive-Orbitrap-MS comprehensively. Halo-C18 column (2.1 mm×100 mm，2.7 μm) was used for gradient elution with 0.05% formic acid aqueous-0.05% acetonitrile formate as mobile phase in positive and negative ion modes. The flow rate was 0.3 mL·min-1，the column temperature was 40 ℃，and the injection volume was 5 μL.Result:The chemical compound in P. frutescens was deduced and identified based on the retention time of chromatography，and the exact molecular weight，excimer ion peaks，fragment ions and reference materials in Xcalibur software. The chemical composition of P. frutescens was identified by Mass Frontier 7.0 software. Totally 4 amino acids，7 phenylpropanoids，10 flavonoids，12 triterpenoids，7 organic acids，4 fatty acids，10 unknown compounds and 54 compounds were identified. Among them，6 triterpene acids, including glochidone, were identified in P. frutescens for the first time. The structures of five characteristic compounds were analyzed. There were 45 constituents in P.frutescens leaves and 32 constituents in P. frutescens stalks. They had 23 common constituents.Conclusion:LC-MS can identify the components of P. frutescens rapidly and effectively. This study provides an important theoretical basis for the quality control of different parts of P. frutescens and the development and utilization of P. frutescens.
