Effect of Coptidis Rhizoma-Zingiberis Rhizoma Extract on Colonic Epithelium TLR4/NF-κB Signaling Pathway in Mice with Ulcerative Colitis
10.13422/j.cnki.syfjx.20200202
- VernacularTitle:黄连-干姜提取物对溃疡性结肠炎小鼠结肠上皮TLR4/NF-κB信号通路的影响
- Author:
Shu-guang YAN
1
;
Yi HUI
1
;
Qian LI
1
;
Jing-tao LI
2
;
Hai-liang WEI
2
Author Information
1. Basic Medical College of Shaanxi University of Chinese Medicine, Xianyang 712046, China
2. Hospital Affiliated to Shaanxi University of Chinese Medicine, Xianyang 712000, China
- Publication Type:Research Article
- Keywords:
ulcerative colitis;
Coptidis Rhizoma-Zingiberis Rhizoma berberine;
6-shogaol;
Toll-like receptors 4 (TLR4)/nuclear factor kappa B (NF-κB) signaling pathway
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(4):70-75
- CountryChina
- Language:Chinese
-
Abstract:
Objective::To observe the effect of berberine and 6-shogaol, main components of Coptiae Rhizoma and Zingiberis Rhizoma, on the inflammatory signaling pathway of Toll-like receptors 4 (TLR4)/nuclear factor kappa B (NF-κB) in colonic epithelial cells of mice with ulcerative colitis. Method::Fifty Kunming mice were randomly divided into normal group, model group, berberine group (100 mg·kg-1), 6-shogaol group (100 mg·kg-1), and 6-shogaol combined with berberine group (200 mg·kg-1), with 10 mice in each group. A mouse model of ulcerative colitis was established through oral administration with 2% dextroan sulfate for two weeks. Each group was given corresponding drugs by gavage, while normal group and model group were given equal amount of normal saline. Serum and colon tissue samples were taken 20 days after administration. Enzyme-linked immunosorbent method was used to detect serum interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α) expressions, and real-time fluorescence quantitative polymerase chain reaction(Real-time PCR) and Western blot method were used to detect TLR4, NF-κB p65 mRNA and protein expressions in colon epithelial tissue. Result::Compared with the normal group, relative expressions of TLR4 and NF-κB p65 mRNA and protein were increased in the model group (P<0.01), and the contents of serum IL-1β and TNF-α were increased (P<0.01). Compared with the model group, relative expressions of TLR4 and NF-κB p65 mRNA and protein were significantly decreased in 6-shogaol group, berberine group and 6-shogaol combined with berberine group (P<0.01), and the contents of serum IL-1β and TNF-α were significantly decreased (P<0.01). Among the three groups, 6-shogaol combined with berberine group had the strongest effect (P<0.01). Conclusion::Both 6-shogaol and berberine can inhibit colonic inflammation, reduce inflammatory damage and treat ulcerative colitis. The combined application of 6-shogaol and berberine has a significant synergism effect. The mechanism is related to the excessive activation of TLR4/NF-κB pathway and the regulation of non-controllable intestinal inflammation.
