Analysis on Mechanism of Adenine-induced Kidney-Yang Deficiency Rats Treated with Different Processed Products of Curculiginis Rhizoma
10.13422/j.cnki.syfjx.20201156
- VernacularTitle:仙茅不同炮制品对腺嘌呤致肾阳虚大鼠的作用机制分析
- Author:
Cheng-guo JU
1
;
Yuan-yuan LI
1
;
Wei WANG
1
;
Tian-zhu JIA
1
;
Xue AI
1
Author Information
1. School of Pharmacy,Chinese Materia Medica Processing Engineering Center of Liaoning Province,Liaoning University of Traditional Chinese Medicine,Dalian 116600,China
- Publication Type:Research Article
- Keywords:
Curculiginis Rhizoma;
processing;
adenine;
invigorating kidney and strengthening Yang;
cytochrome P450 3A (CYP3A);
kidney-Yang deficiency;
hypothalamus-pituitary-target gland axis
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(16):101-107
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study on the mechanism of invigorating kidney and strengthening Yang of different processed products of Curculiginis Rhizoma aqueous extracts in rats with kidney-Yang deficiency induced by adenine. Method:Taking Guifu Dihuang pills as the positive drug group (the dosage of 2.466 g·kg-1), after intragastric administration for 4 weeks, enzyme-linked immunosorbent assay (ELISA) was used to compare the effects of different processed products of Curculiginis Rhizoma aqueous extracts (the dosage of 2.742 g·kg-1) on the levels of thyroid stimulating hormone (TSH), 17-hydroxycorticosteroids (17-OHCS), estradiol (E2), testosterone (T), triiodothyronine (T3), thyroxine (T4) and cortisol (COR) in serum of rats with kidney-Yang deficiency induced by adenine. The activity of cytochrome P450 3A (CYP3A) in rat liver and kidney microsomes was determined by Nash colorimetry. Result:All the processed products aqueous extracts could improve the function of hypothalamus-pituitary-target gland axis in rats with kidney-Yang deficiency induced by adenine, and the total score was in the order of Euodiae Fructus juice-processed products (29 points)>salt-processed products (25 points)>rice wine-processed products (23 points)>raw products (17 points)>Zingiberis Rhizoma juice-processed products (11 points). And the different processed products of Curculiginis Rhizoma could increase CYP3A activity of liver and kidney microsomes of kidney-Yang deficiency rats, especially the Euodiae Fructus juice-processed products and salt-processed products. Conclusion:All the processed products of Curculiginis Rhizoma can effectively treat the syndrome of kidney-Yang deficiency in rats, among them, Euodiae Fructus juice-processed products and salt-processed products have more significant effect on invigorating kidney and strengthening Yang.
