Analysis of Chemical Variability on Different Processed Products of Glycyrrhiza uralensis Dry Roots and Rhizomes Based on UPLC-Q-TOF-MSE and Plant Metabolomics
10.13422/j.cnki.syfjx.20201073
- VernacularTitle:基于液质联用技术和植物代谢组学的甘草炮制品化学成分差异性分析
- Author:
Wang WEN
1
;
Li LI
2
;
De-kun LI
2
;
Lei ZHANG
2
;
Da-zheng ZHOU
3
;
Yue-wu YANG
3
;
Jian-Hui YANG
3
;
Bo-yang YU
1
;
Ai-chun JU
2
Author Information
1. School of Traditional Chinese Pharmacy,China Pharmaceutical University,Nanjing 211198,China
2. Tianjin Tasly Pride Pharmaceutical Co. Ltd.,Tianjin 300410,China
3. Tasly Holding Group Co. Ltd.,Tianjin 300410,China
- Publication Type:Research Article
- Keywords:
Glycyrrhiza uralensis dry roots and rhizomes;
processed products;
plant metabolomics;
quality differential markers;
ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MSE);
chemical compositions;
liquiritin
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(17):104-110
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To identify the quality differential markers of different processed products of Glycyrrhiza uralensis dry roots and rhizomes. Method:Ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MSE) was used to collect high-precision mass-charge ratio and ion response strength information of the components in G. uralensis dry roots and rhizomes before and after processing by negative ion mode. The data set collected after pretreatment was analyzed with principal component analysis (PCA) and orthogonal partial least squares-discriminant analysis (OPLS-DA) to quickly search the differential components in different processed products of G. uralensis dry roots and rhizomes. Differential components were identified according to the relative molecular weight, fragment ion, mass spectrum database and related literature information, then the migration of components before and after processing was studied. Result:A total of 10 quality differential markers were searched from raw products, roasted products and honey-roasted products of G. uralensis dry roots and rhizomes, mainly derivatives of liquiritin and glycyrrhizic acid. Among them, the contents of 6''-O-acetylliquiritin apioside, 6''-O-acetylliquiritin apioside isomer, 6''-O-acetylliquiritin, formononetin and 11-deoxo-18β-glycyrrhetic acid were the highest in the raw products, the contents of 6''-O-acetylisoliquiritin apioside, 6''-O-acetylisoliquiritin, isoliquiritin and glycyrrhetic acid 3-O-glucuronide were the highest in the roasted products, the content of liquiritin was the lowest in the honey-roasted products. Conclusion:There are some chemical differences among the three products. This study can provide material basis for the quality control and pharmacodynamic research of processed products of G. uralensis dry roots and rhizomes.
