Effect of Zuoguiwan on Expression of Ca2+ Transport Pathway-related Protein in Ovariectomized Rats
10.13422/j.cnki.syfjx.20192206
- VernacularTitle:左归丸对去势大鼠钙转运通路相关蛋白的影响
- Author:
Hao TANG
1
;
Na YU
2
;
Xiang-zhong QIU
1
;
Zhe-xiang WANG
1
Author Information
1. Hunan Academy of Traditional Chinese Medicine Affiliated Hospoital, Changsha 410006, China
2. Hunan University of Chinese Medicine, Changsha 410208, China
- Publication Type:Research Article
- Keywords:
Zuoguiwan;
osteoporosis;
transient receptor potential vanilloid receptor V5(TRPV5);
calcium-binding protein(CaBP)-D28K;
Na+ /Ca2+ exchanger 1(NCX1);
plasma membrane Ca2+ -ATPase 1b(PMCA1b)
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(2):13-18
- CountryChina
- Language:Chinese
-
Abstract:
Objective::To explore the effect of Zuoguiwan on the bone mineral density (BMD) and the expressions of Ca2+ transport-associated protein in ovariectomized rats. Method::The 48 female SD rats were randomly divided into six groups: normal group, model group, sham operation group, estrogen group(0.167 mg·kg-1) and low and high-dose Zuoguiwan groups(9.6, 38.4 g·kg-1), with 10 rats in each group. Except for the sham-operated group, the ovariectomized rats in the other groups received the bilateral ovariectomy. Therapeutic intervention was given in each group for 3 months after the establishment of the model. After 12 weeks, BMD was measured using dualenergy X-ray absorptiometry. Tartrated presistant acid phosphatse(TRACP) and serum calcium were detected by biochemical kits.Protein expression in Ca2+ transport (Bone tissue) was detected by Western blot. Result::Compared with the normal group, the serum calcium of the model group was decreased(P<0.01). Compared with the normal group, BMD of the model group was decreased (P<0.01). The serum calcium of rats in high-dose group and western medicine group was higher than that of model group(P<0.01). BMD in model group was lower than that of Zuoguiwan groups and estrogen group(P<0.05). There was no significant difference in TRACP among the groups. Nilestriol and Zuoguiwan can down-regulate the expressions of TRPV5, NCX1, CaBP-D28K and PMCA1b in bone tissue of castrated rats(P<0.05, P<0.01). Conclusion::Zuoguiwan can down-regulate the expressions of Ca2+ transport-associated proteins (Bone tissues) in rat osteoclasts, with an efficacy on osteoporosis.
