ApoE gene polymorphism in patients with intracerebral hemorrhage at different ages and its relationship with blood lipid level
10.3760/cma.j.issn.1008-6706.2020.09.013
- VernacularTitle:不同年龄段脑出血患者的载脂蛋白E基因多态性及其与血脂水平的关系
- Author:
Jianfeng BAI
1
;
Liang YU
;
Qiangbo SUI
Author Information
1. 山东省，威海市中心医院神经外科 264400
- From:
Chinese Journal of Primary Medicine and Pharmacy
2020;27(9):1085-1089
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study ApoE gene polymorphism in patients with intracerebral hemorrhage at different ages and its relationship with blood lipid level.Methods:From December 2017 to December 2019, 360 inpatients with cerebral hemorrhage received by neurosurgery department of Weihai Central Hospital were selected as the cerebral hemorrhage group.According to the age of patients, patients with cerebral hemorrhage were divided into low age group (age<30 years), middle age group (30 years≤age≤60 years) and high age group (age>60 years), with 120 cases in each group.Another 120 healthy persons who received health examination during the same period were selected as control group.The levels of high density lipoprotein (HDL), low density lipoprotein (LDL), triglyceride (TG) and total cholesterol (TC) in peripheral blood were measured.ApoE gene polymorphism was determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).Results:There were no statistically significant differences in sex, age, body mass index between the cerebral hemorrhage group and the control group (χ 2=0.045, t=0.459, t=0.655, P=0.832, 0.323, 0.256). The systolic blood pressure, diastolic blood pressure, history of hypertension, history of diabetes, smoking and drinking in the cerebral hemorrhage group were significantly higher than those in the control group ( t=12.598, t=13.003, χ 2=49.308, χ 2=10.246, χ 2=45.562, χ 2=8.602, P=0.000, 0.000, 0.000, 0.000, 0.000, 0.003). There were statistically significant differences in TC, TG, LDL and HDL among the control group, the younger group, the middle-aged group and the older group ( F=36.893, 131.263, 808.908, 143.188, all P=0.000). The highest genotype frequency was E3/3 in the control group, the younger group, the middle-aged group and the older group(70.00%, 68.33%, 68.33% and 66.67%). There was no statistically significant difference in genotype frequencies of ApoE among the groups (χ 2=2.025, 1.524, 0.693, 0.308, 0.815, 2.025, P=0.567, 0.677, 0.875, 0.959, 0.856, 0.567). The highest allele frequencies of ApoE in the control group, the younger group, the middle-aged group and the older group were epsilon 3 (81.66%, 81.66%, 79.99%, 80.83%). There was no statistically significant difference in the allele frequencies of ApoE among the groups (χ 2=0.061, 0.125, 0.241, P=0.996, 0.989, 0.971). For patients with E3/3 genotype cerebral hemorrhage, the levels of TC, TG, LDL and HDL in the younger age group were (4.43±0.33)mmol/L, (1.50±0.37)mmol/L, (2.58±0.32)mmol/L and (1.53±0.32)mmol/L, respectively.The TC, TG, LDL and HDL levels in middle-aged group were (4.22±0.34)mmol/L, (1.87±0.32)mmol/L, (3.30±0.31)mmol/L and (1.85±0.35)mmol/L, respectively.The TC, TG, LDL and HDL levels in the elderly group were (3.85±0.35)mmol/L, (2.09±0.35)mmol/L, (3.60±0.33)mmol/L and (2.13±0.34)mmol/L, respectively.There were statistically significant differences in TC, TG, LDL and HDL among all groups ( F=60.289, 59.844, 218.120, 64.364, all P=0.000). Conclusion:There is no significant correlation between ApoE gene polymorphism and the occurrence of widespread intracerebral hemorrhage in different age groups.There is no correlation between E3/3 genotype and blood lipid level in patients with intracerebral hemorrhage in different age groups.
