Effects of allogeneic bone marrow mesenchymal stem cells on the polarization of peritoneal macrophages in rats with acute necrotizing pancreatitis
10.3760/cma.j.cn115667-20190918-00081
- VernacularTitle:同种异体骨髓间充质干细胞对急性坏死性胰腺炎大鼠腹腔巨噬细胞极化的影响
- Author:
Lin WANG
1
;
Ping XU
;
Jing WANG
;
Jun TIAN
;
Xiaolan XIE
Author Information
1. 南京医科大学上海松江临床医学院，上海市松江区中心医院消化内科，上海 200090
- From:
Chinese Journal of Pancreatology
2020;20(2):120-125
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of bone marrow mesenchymal stem cells (BMSCs) on the inflammatory response and polarization of peritoneal macrophages in rats with acute necrotizing pancreatitis (ANP).Methods:Thirty Sprague Dawley male rats were divided into control group, ANP group, low-dose (0.5×10 6 cells), medium-dose(1×10 6 cells)and high-dose (2×10 6 cells)BMSCs treatment groups. ANP model was established via the retrograde injection of 1ml/kg 5% sodium taurocholate hydrate into pancreaticobiliary duct. BMSCs were injected via caudal vein 1 h after molding. Tissues of the rat pancreas were taken for pathological examination, and iNOS and ARG1 positive macrophages were detected by immunohistochemistry. The level of amylase, TNF-α, IL-1β and MPO were measured in peripheral blood. Abdominal macrophages were obtained by intraperitoneal perfusion, and TNF-α and iNOS mRNA expression of macrophages were detected by real-time fluorescence quantitative PCR; and the dynamic changes of M1(F4/80 + , CCR2 + ) and M2 (F4/80 + , CD 163+ ) macrophages were detected by flow cytometry. Results:The pathological injury of pancreatic tissue in the three treatment groups was obviously less serious than that of ANP group. In ANP group, more iNOS and ARG1 positive macrophages were present in rat pancreatic tissue, while none or only few iNOS and ARG1 positive macrophages were observed in control group and treatment group. The therapeutic effects of medium dose (1×10 6)BMSCs treatment group was significantly better than those of the other two treatment groups, with statistically significant differences(all P<0.05). Compared with ANP group, TNF-α[ (99.5±11.8)ng/L vs (185.5±27.5)ng/L ], IL-1β[ (24.1±3.7)ng/L vs (128.5±66.1)ng/L ], MPO [ (643.8±98.4)ng/L vs (2131.9±261.4)ng/L ], TNF-α (2.16±0.98 vs 6.53±3.45) and iNOS (2.32±1.88 vs 7.37±2.98) mRNA expression in peritoneal macrophages, and the ratio of M1/M2 (1.53±0.10 vs 2.02±0.31)were all greatly decreased, and the differences were all statistically significant (all P<0.05), except for blood amylase. All the above indexes in ANP group and the three treatment groups were all significantly higher than those in the control group, with statistically significant differences (all P<0.05). Conclusions:Intravenous infusion of allogeneic BMSCs can inhibit the M1 type polarization of peritoneal macrophages, reduce the M1/M2 ratio of peritoneal macrophages, and alleviate the inflammatory response in ANP rats.
