Effect of rehabilitation training combined with bailing capsule on the efficacy and pulmonary function of patients with chronic obstructive pulmonary disease via RhoA/Rho-kinase signaling pathway
10.3760/cma.j.cn115455-20191114-00915
- VernacularTitle:呼吸康复训练联合百令胶囊通过人RAS同源基因家族成员A/Rho-kinase信号通路对慢性阻塞性肺疾病患者的疗效及肺功能的影响
- Author:
Juanjuan WU
1
;
Chunxia WANG
;
Bin XING
;
Yanling JIANG
;
Shuo CHEN
Author Information
1. 陆军第八十一集团军医院呼吸内科，河北张家口 075000
- From:
Chinese Journal of Postgraduates of Medicine
2020;43(11):1035-1039
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of respiratory rehabilitation training combined with bailing capsule on the efficacy and lung function of patients with chronic obstructive pulmonary disease (COPD) through RhoA/Rho-kinase signaling pathway.Methods:One hundred patients with COPD from February 2016 to September 2017 in the Eighty-Eleventh Army Hospital of Hebei Army were randomly divided into control group (50 cases) and bailing capsule + respiratory rehabilitation training group (50 cases). At the same time, 50 healthy people were selected as blank group. The patients in the control group were treated with bailing capsule, and patients in the combined treatment group were treated with respiratory rehabilitation training combined with bailing capsule. The pulmonary function (FEV 1, FVC, FEV 1/FVC%) and clinical efficacy were analyzed. The expression of RhoA, Rock Ⅰ/Ⅱ protein and mRNA were detected by immunoblotting and qRT-PCR. Results:After treatment, the indexes of pulmonary function in the combined group were significantly higher than those in the control group: (2.34 ± 0.91) L vs. (1.91 ± 0.83) L, (2.98 ± 0.83) L vs. (2.34 ± 0.86) L, (79.63 ± 9.95)% vs. (76.13 ± 6.97)% ( P < 0.05). Compared with the blank group, RhoA mRNA and Rock Ⅰ/Ⅱ mRNA in the combined treatment groups were significantly higher than those in the control group ( P < 0.05), and RhoA mRNA and Rock Ⅰ/Ⅱ mRNA in the combined group were significantly lower than those in the control group ( P < 0.05). Compared with the blank group, the RhoA mRNA and Rock Ⅰ/Ⅱ proteins combined treatment groups were significantly higher than those in the control group ( P < 0.05), and the RhoA mRNA and Rock Ⅰ/Ⅱ proteins in the combined group were significantly lower than those in the control group (2.46 ± 0.18 vs. 4.38 ± 0.21, 1.72 ± 0.11 vs. 2.36 ± 0.24, 1.79 ± 0.24 vs. 3.34 ± 0.21) ( P < 0.05). After treatment, the 6MWD and the total clinical effective rate in the combined group were significantly better than those in the control group: 92.00% (46/50) vs. 78.00% (39/50) ( P < 0.05). Conclusions:Respiratory rehabilitation training combined with bailing capsule can significantly improve the pulmonary function of COPD patients, improve the clinical efficacy, and can down-regulate the expression of RhoA, Rock Ⅰ/Ⅱ protein and mRNA through RhoA/Rho-kinase signaling pathway.
