Epidemiological survey and serological characteristic analysis of Epstein-Barr virus infection in the children
10.3760/cma.j.cn101070-20190524-00449
- VernacularTitle:儿童EB病毒感染的流行病学调查及血清学特征分析
- Author:
Bo WANG
1
;
Ying QI
;
Qiang RUAN
Author Information
1. 中国医科大学附属盛京医院病毒研究室，沈阳 110004
- From:
Chinese Journal of Applied Clinical Pediatrics
2020;35(18):1403-1406
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the epidemic situation of Epstein-Barr virus (EBV) infection in children in Shenyang and analyze the serological characteristics.Methods:The IgM antibody against EBV capsid antigen (VCA-IgM), IgG antibodies against capsid antigen (VCA-IgG), nuclear antigen IgG (EBNA-IgG) and early antigen (EA-IgG) of 26 714 pediatric patients hospitalized in Shengjing Hospital, China Medical University from August 2017 to July 2018 were detected simultaneously by LIAISON chemiluminescence analyzer.The infection stages of EBV were determined comprehensively according to the antibody spectrum of VCA-IgM, VCA-IgG and EBNA-IgG.Meanwhile, EBV antibodies were re-examined after 4-6 weeks in the patients with positive VCA-IgM only.Results:Among the 26 714 patients, a total of 2 963 cases (11.09%) were VCA-IgM positive, 15 349 cases (57.46%) were VCA-IgG positive, 14 263 cases (53.39%) were EBNA-IgG positive and 731 cases (2.74%) were EA-IgG positive.The positive rates of VCA-IgM, VCA-IgG, EBNA-IgG and EA-IgG in 15 149 boys were 10.98% (1 663/15 149 cases), 57.73% (8 745/15 149 cases), 53.49% (8 103/15 149 cases) and 2.57% (389/15 149 cases), respectively.The positive rates of VCA-IgM, VCA-IgG, EBNA-IgG and EA-IgG in 11 565 girls were 11.24% (1 300/11 565 cases), 57.10% (6 604/11 565 cases), 53.26% (6 160/11 565 cases) and 2.96% (342/11 565 cases), respectively.There was no significant difference in the positive rates of the 4 antibodies between male and female(all P>0.05). The positive rates of VCA-IgG and EBNA-IgG increased gradually with the age of children ( χ2=4 057.744, 4 776.285, all P<0.05), and those of VCA-IgG and EBNA-IgG were 87.98%(1 317/1 497 cases) and 88.78%(1 329/1 497 cases) respectively when children reached 14 years old.Typical primary infection [36.38%(1 078/2 963 cases)] and the recovery or reactivation of primary infection[43.81%(1 298/2 963 cases)] were predominant in acute infection children with positive VCA-IgM.Moreover, 95.09% (12 777/13 437 cases) of the previously infected cases had both positive VCA-IgG and positive EBNA-IgG.Of the 198 children with only positive VCA-IgM, 133(67.17%) did not show seroconversion.There was no case who had only positive EA-IgG, and 3.19% (428/13 437 cases) previously infection cases had positive EA-IgG. Conclusions:The positive rates of EBV 4 antibodies are not different between male and female children, and the past infection rate increases with age.The combined detection of antibodies can provide more detailed and reliable information for clinical diagnosis.Double serum antibodies detection can improve diagnosis accuracy; EA-IgG is an important serological marker for diagnosing reactivation of EBV infection.
