Change of Notch1 expression level on the surface of myeloma cells in multiple myeloma patients and its clinical significance
10.3760/cma.j.cn.115356-20190819-00159
- VernacularTitle:多发性骨髓瘤患者骨髓瘤细胞表面Notch1表达水平变化及其临床意义
- Author:
Honghui YE
1
;
Shuping ZHONG
;
Xuegang LI
Author Information
1. 中山大学附属第五医院血液科，珠海 519000
- From:
Journal of Leukemia & Lymphoma
2020;29(5):261-264
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the dynamic change of Notch1 expression level on the surface of myeloma cells in patients with multiple myeloma (MM) and its clinical significance.Methods:A total of 38 patients with MM in the Fifth Affiliated Hospital of Sun Yat-Sen University from April 2013 to July 2019 were selected. At the same time, 18 patients with mild iron deficiency anemia were selected as controls. Flow cytometry was used to detect Notch1 expression levels on the surface of myeloma cells for MM patients at the first diagnosis (before the treatment), in remission stage (the time of best effect during the treatment) and in the advanced stage (disease progression), and on the surface of normal plasma cells of the control group. The therapeutic efficacy was analyzed, and the expression levels of Notch 1 were compared among the different stages of MM patients and the control group.Results:Among 38 MM patients, 6 cases (15.8%) had strict complete remission (sCR), 14 cases (36.8%) had complete remission (CR), 10 cases (26.3%) had very good partial remission (VGPR), 6 cases (15.8%) had partial remission (PR), 2 cases (5.3%) died after the first course of treatment. Notch 1 detection was performed in 36 patients. The expression rate of Notch1 on the surface of myeloma cells at the first diagnosis, in remission stage, and in the advanced stage of MM patients and on the surface of normal plasma cells of the control group was (13.1±3.9)%, (2.7±1.2)%, (5.4±1.7)% and (2.1±0.3)%; the difference was statistically significant ( F = 151.4, P < 0.01). The expression rate of Notch1 in MM patients at the first diagnosis and in advanced stage was higher than that in remission stage (all P < 0.01), while the expression rate of Notch1 on the surface of myeloma cells in remission stage was higher than that on the surface of normal plasma cells in the control group ( t = 2.219, P = 0.038). Conclusion:The Notch1 signaling pathway may play an important role in the occurrence and development of MM, and Notch1 detection has a certain guiding significance for the monitoring, treatment and prognosis assessment of MM.
