Evaluation of Different Effect of Pith-nodecayed and Pith-decayed Products of Scutellariae Radix on Large Intestine Damp-heat Syndrome Based on Pharmacological Indexes
10.13422/j.cnki.syfjx.20192154
- VernacularTitle:基于药理指标评价子芩与枯芩对大肠湿热证的作用差异
- Author:
Fang-lei ZHANG
1
;
Jia-wen ZHAO
1
;
Jiang-hui HU
1
;
Kun-yu XIAO
1
;
Ying XIE
1
;
Lin LEI
1
;
Yan-ju LIU
1
Author Information
1. Engineering Research Center of Processing Technology of Traditional Chinese Medicine in Hubei Province, School of Pharmacy, Hubei University of Chinese Medicine, Wuhan 430065, China
- Publication Type:Research Article
- Keywords:
Scutellariae Radix;
pith-nodecayed products of Scutellariae Radix;
pith-decayed products of Scutellariae Radix;
large intestine damp-heat syndrome;
partial least squares-discriminant analysis;
inflammatory factors;
secretory immunoglobulin A
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(7):57-64
- CountryChina
- Language:Chinese
-
Abstract:
Objective::To compare the pharmacodynamic effects of pith-nodecayed and pith-decayed products of Scutellariae Radix on rats with large intestine damp-heat syndrome, and to demonstrate the scientificness of dividing Scutellariae Radix into pith-nodecayed and pith-decayed products as medicines by modern pharmacological test. Method::Rats were randomly divided into blank group, model group, low-and high-dose group of pith-nodecayed products (0.9, 3.6 g·kg-1), low-and high-dose group of pith-decayed products (0.9, 3.6 g·kg-1), Scutellariae Radix group (0.9 g·kg-1), compound berberine tablets group (positive drug group, 0.045 g·kg-1), and 8 rats in each group. Taking model rats with large intestine damp-heat syndrome, the body temperature, thymus index, spleen index, pathological sections of colon and ileum, inflammatory factors and Secretory immunoglobulin (SIg) A content were selected as indexes to evaluate the therapeutic effect of pith-nodecayed and pith-decayed products on large intestine damp-heat syndrome, and make comprehensive evaluation of the difference in efficacy between them. Partial least squares-discriminant analysis (PLS-DA) was employed to analyze the pharmacological indexes of these two products against large intestine damp-heat syndrome. Result::Pith-nodecayed and pith-decayed products of Scutellariae Radix with different doses could reduce the body temperature, thymus index, spleen index, contents of interleukin (IL)-2, IL-6, IL-1β in serum and SIgA content in intestinal mucosa, and most of them had significant differences (P<0.05, P<0.01). Compared with the isodose group of pith-decayed products, the effect of corresponding dose group of pith-nodecayed products was better, and most of them had significant differences (P<0.05, P<0.01). PLS-DA results indicated that there were significant differences in the pharmacological effects of pith-nodecayed and pith-decayed products, and they were clustered on one side, respectively. Conclusion::Both of pith-nodecayed and pith-decayed products of Scutellariae Radix have therapeutic effect on large intestine damp-heat syndrome with distinctly different strength of action, and pith-nodecayed products is superior to pith-decayed products, which verify the scientific nature of pith-nodecayed products was specializedly used to treat bowel disease in ancient times.
