Changes of cardiac function and oxidative stress in sepsis rats
10.13929/j.issn.1672-8475.2020.07.010
- VernacularTitle: 脓毒症大鼠心功能及氧化应激水平变化
- Author:
Zhen WANG
1
Author Information
1. Department of Clinical Medicine, School of Medicine, Shihezi University
- Publication Type:Journal Article
- Keywords:
Echocardiography;
Oxidative stress;
Rats;
Sepsis
- From:
Chinese Journal of Interventional Imaging and Therapy
2020;17(7):425-429
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the changes of echocardiography, histopathological manifestations and oxidative stress of myocardium in sepsis rats. Methods: Forty-eight 12-week-old SD rats were randomly divided into 4 groups, i.e. cecal ligation and perforation 24 h group (CLP24 h group) and 48 h group (CLP48 h group), sham operation group (Sham group) and Blank group (each n=12). Color Doppler ultrasound was used to measure the left ventricular end-diastolic diameter (LVEDD), interventricular septum thickness at end-diastole (IVSD), left ventricular posterior wall thickness at end-diastole (LVPWD), left ventricular fraction shortening (LVFS), left ventricular ejection fraction (LVEF) and heart rate (HR). HE and Masson staining of myocardial tissues were performed to observe the pathological changes of heart. The levels of superoxide dismutase (SOD), reduced glutathione (GSH) and malondialdehyde (MDA) in myocardial tissues were measured, and the correlations with LVEF were analyzed. Results: Statistical differences of LVEDD, IVSD, LVFS, LVEF and HR were found among 4 groups (all P<0.01). LVFS, LVEF and HR in Blank group were all larger than those in Sham group, CLP24 h group and CLP48 h group in turn (all P<0.05). Some myocardial cells in CLP24 h group and CLP48 h groups were swollen and necrotic, while collagen fibers in myocardial space increased significantly and became more obvious in CLP48 h group (all P<0.05). SOD and GSH in Blank group were higher than those in Sham group, CLP24 h group and CLP48 h group in turn, while MDA in Blank group was lower than in Sham group, CLP24 h group and CLP48 h group in turn. LVEF was positively correlated with SOD and GSH (r=0.922 6, 0.938 0, both P<0.001) and negatively correlated with MDA (r=-0.929 8, P<0.001). Conclusion: Sepsis can cause abnormalities of function and changes of structure in rat heart. Oxidative stress may be important mechanism for sepsis-induced heart damage.
