Study on Intestinal Flora and Expression of NOX1 in Patients With Irritable Bowel Syndrome
10.3969/j.issn.1008-7125.2019.09.004
- Author:
Shuting SHENG
1
Author Information
1. Department of Gastroenterology, Peace Hospital Affiliated to Changzhi Medical College
- Publication Type:Journal Article
- Keywords:
Intestinal Flora;
Irritable Bowel Syndrome;
NADPH Oxidase 1
- From:
Chinese Journal of Gastroenterology
2019;24(9):528-533
- CountryChina
- Language:Chinese
-
Abstract:
Background: Studies have shown that intestinal flora and nicotinamide-adenosine dinucleotide phosphate oxidase 1 (NOX1) play important roles in the occurrence of chronic visceral pain, however, the roles in irritable bowel syndrome (IBS) are not clear. Aims: To study the correlation of intestinal flora mucosal characteristics with NOX1 expression in patients with IBS. Methods: Colonic mucosa specimens of IBS patients diagnosed by Rome criteria and healthy subjects from July 2017 to December 2017 at Peace Hospital Affiliated to Changzhi Medical College were collected. High-throughput sequencing was used to detect intestinal mucosal flora, and the species diversity and abundance of the intestinal flora were analyzed. The expression of NOX1 in colonic mucosa was determined by immunohistochemistry. Results: The intestinal mucosal flora of IBS group was mainly composed of Bacteroides, Proteobacteria and Firmicutes. There was no significant difference in diversity of flora among IBS-D group, IBS-U group and control group (P>0.05). Compared with the control group, the abundance of Tissierella was significantly increased in the IBS group (H=6.688, P<0.05), however, the abundance of Granulicatella was significantly decreased (H=6.212, P<0.05). Compared with the control group and IBS-U group, the abundances of Porphyromonas, Marmoricola, Cardiobacterium in IBS-D group were significantly increased (P all <0.05). Compared with the control group, the expression of NOX1 was significantly increased in IBS-U group and IBS-D group (P<0.01). Colonic mucosal NOX1 expression was positively correlated with Tissierella abundance (r=0.611, P<0.05), but not with Granulicatella abundance (r=0.253, P=0.376). There were no significant correlations between Tissierella, Granulicatella abundances, colonic mucosal NOX1 expression and severity of IBS (P>0.05). Conclusions: Compared with healthy subjects, there is no significant change in the diversity of intestinal mucosal flora in IBS patients. The abundance of some flora is changed, and the change of abundance of flora varies in different subtypes. The expression of NOX1 in colonic mucosa of IBS patients is up-regulated, and there was a certain correlation with the abundance of Tissierella.
