Changes of Gut Microbiota Composition in Elderly People Based on High-throughput Sequencing
10.3969/j.issn.1008-7125.2019.09.002
- Author:
Li YU
1
Author Information
1. Department of Domestic Inpatient, Health Management Institute, Chinese PLA General Hospital
- Publication Type:Journal Article
- Keywords:
Aged;
Gut Microbiota;
High-Throughput Nucleotide Sequencing
- From:
Chinese Journal of Gastroenterology
2019;24(9):517-523
- CountryChina
- Language:Chinese
-
Abstract:
Background: With the trend of aging of Chinese population, the healthcare of the elderly has attracted much attention. Aims: To study the impact of aging on gut microbiota composition for the sake of achieving health promotion in the elderly via regulation of gut microbiota. Methods: Thirty healthy elderly subjects ( 60-80 years) and 10 healthy young subjects (18-40 years) were enrolled and the fecal samples were collected. High-throughput sequencing was performed for determining the sequence of 16S rRNA gene V3-V4 region of all fecal bacteria. Then MiSeq 16S metagenomic APP and Qimme software were used to analyze the classification and diversity index of gut microbiota, the significant differences between sample groups and the biomarkers for each group. Results: The mean OTU and diversity of gut microbiota between healthy elderly group and healthy young group were similar (P>0.05). Metastats analysis showed that there were no significant differences between the two groups in dominant phyla, including Bacteroidetes, Firmicutes and Proteobacteria (P>0.05). At the genus level, more than 20 genera had statistically significant differences between the two groups (P<0.05). The abundance of Sarcina, Anaeroplasma, Methylobacterium, Comamonas, Olsenella, Rheinheimera, Terrisporobacter, Acidovorax, Coriobacteriaceae_UCG-003, Coprobacillus, Gelria, Aquabacterium, Acidaminococcus, Caldicoprobacter, Enterorhabdus and Turicibacter were significantly increased in healthy elderly group, while the abundance of Acetanaerobacterium, Vagococcus, Ruminococcaceae_UCG-009, Oxalobacter and Coprococcus_2 were significantly increased in healthy young group. LEfSe analysis showed that the biomarkers of healthy elderly group were enriched in Acidovorax, Eubacterium_nodatum_group, Clostridiaceae_1 and Comamonadaceae, and those of the healthy young group were concentrated in Prevotella_2, Roseburia and Acetanaerobacterium. Conclusions: The composition and function of gut microbiota change with aging to some extent. Butyrate-producing bacteria are significantly reduced whereas the opportunistic pathogens are increased in the elderly.
