Mutation in p53 Tumor Suppressor Gene in Gestational Trophoblastic Neoplasia.
- Author:
Seung Cheol KIM
1
;
Hye Sung MOON
;
Hyung Rae KIM
Author Information
1. Department of Obstetrics and Gynecology, College of Medicine, Molecular Section, Ewha Medical Research Center, Ewha Womans University, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
p53 gene;
Gestational Trophoblastic Neoplasia
- MeSH:
Choriocarcinoma;
Ethidium;
Exons;
Female;
Genes, p53;
Genes, Tumor Suppressor*;
Gestational Trophoblastic Disease*;
Hydatidiform Mole;
Polymerase Chain Reaction;
Polymorphism, Single-Stranded Conformational;
Pregnancy
- From:Korean Journal of Gynecologic Oncology and Colposcopy
1997;8(4):339-349
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
This study attempted to determine the status of tumor suppressor gene p53 in gestational trophoblstic neoplasia. In this study, 19 cases of hydatidiform moles and two choriocarcinoma cell lines(JAR, JEG-3) were evaluated for the presence of alterations of p53 gene. The p53 mutations were evaluated by nonisotopic olymerase chain reaction-single strand conformation polymorphism(PCR-SSCP) and automatic sequencing of PCR products in exons 5, 6, 7, 8. But we could not find any mutations of the p53 gene. This results suggest that p53 mutation maybe rarely involved in the pathogenesis of gestational trophoblastic neoplasia. And nonisotopic SSCP analysis by ethidium bromide staining is considered as an useful method for detecting single base differences in PCR products.
