Micro PET/CT molecular imaging observation on mechanism of anti-myocardial ischemia/reperfusion injury action of
10.13929/j.issn.1003-3289.2020.07.002
- VernacularTitle: Micro PET/CT分子显像观察广枣-7味丸抗缺血/再灌注损伤作用机制
- Author:
Guojian ZHANG
1
Author Information
1. Department of Nuclear Medicine, The Affiliated Hospital of Inner Mongolia Medical University
- Publication Type:Journal Article
- Keywords:
Guangzao Qiwei pills;
Myocardial ischemia;
Myocardial reperfusion;
Positron-emission tomography;
Tomography, X-ray computed
- From:
Chinese Journal of Medical Imaging Technology
2020;36(7):966-970
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the mechanism of anti-myocardial ischemia/reperfusion injury (MIRI) action of Guangzao Qiwei pills in rats using micro PET/CT molecular imaging. Methods: Totally 120 male SD rats were randomly divided into high dose group, medium dose group and low dose group of Guangzao Qiwei pills, as well as blank control group, model control group and positive control group (each n=20). Micro PET/CT imaging was performed before and 24 h, 48 h, 72 h after model establishment. Volume of interest (VOI) and the mean standardized uptake value (SUVmean) in ischemic region at each time point were calculated. Serum oxidative stress parameters (superoxide dismutase [SOD] and malondialdehyde [MDA]) and marker enzymes of myocardial injury (lactate dehydrogenase [LDH] and creatine kinase [CK]) were observed. All rats were sacrificed after the ending of imaging, and then myocardial pathological changes were observed. Results: Different degrees of imaging agent sparsity-defect were observed in each dose group 24 h and 48 h later. The imaging agent sparsity was still observed in low dose group which recovered in medium and high dose groups. SUVmean of ischemic region gradually increased with time in all 3 dose groups and metabolism gradually recovered (F=121.82, 450.82, 435.75, all P<0.05). Each dose group can Free radicals were eliminated and the damage of myocardial injury reduced in all 3 dose groups, better effect on SOD, on MDA and LDH or on CK were observed in the high dose, the medium dose and the low dose group, respectively (all P<0.05). Conclusion: The mechanism of anti-MIRI action of Guangzao Qiwei Pills achieve by eliminating free radicals in the body, reducing oxidative stress injury and affecting the activities of relevant enzymes. All dose Guangzao Qiwei Pills can protect rats from MIRI, high dose has the best effect. PET/CT molecular imaging allows non-invasive, in vivo and dynamic detection of MIRI and accurately evaluation on the protective role of Guangzao Qiwei Pills in MIRI.
