Energy spectral CT in evaluation on efficacy of Bevacizumab against angiogenesis in rat models of C6 glioma
10.13929/j.issn.1003-3289.2020.07.001
- VernacularTitle: 能谱CT评价贝伐单抗抗大鼠C6胶质瘤血管生成效果
- Author:
Lei HAN
1
Author Information
1. Department of Radiology, Lanzhou University Second Hospital, Lanzhou Second Clinical School,Lanzhou University, Key Laboratory of Medical Imaging of Gansu Province
- Publication Type:Journal Article
- Keywords:
Angiogenesis inhibitors;
Glioma;
Rats;
Tomography, X-ray computed
- From:
Chinese Journal of Medical Imaging Technology
2020;36(7):961-965
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the feasibility and value of quantitative parameters of energy spectral CT for evaluating the efficacy of Bevacizumab against angiogenesis of C6 glioma rat models. Methods: C6 glioma rat models were established through intracranial injection of C6 cell suspension in 30 SD rats. The tumor formation was observed with energy spectrum CT 2 weeks after modeling (day 0). The rats were randomly divided into experimental group and control group and given celiac injection of Bevacizumab injection 5 mg/kg and equal dose of concentration 0.9% saline every day of 1 week, respectively. Spectral CT scanning was performed on the 4th and 8th day after treatment, respectively, and CT values at 40-140 keV and iodine content of tumor were measured and compared. At the end of each scanning, 3 rats of each group were randomly selected and sacrificed, and brain tissue were harvested for HE staining and immunohistochemical staining of vascular endothelial growth factor (VEGF). The differences of the amount of VEGF-positive cells at different time points were compared between 2 groups, and the correlations of spectral CT quantitative parameters and the amount of VEGF-positive cells were analyzed. Results: Totally 26 rats were included, 13 in experimental group and 13 in control group. There was no statistical difference of CT value of 70 keV single energy nor iodine concentration between 2 groups on day 0 (t=1.35, -0.99, both P>0.05). On the 4th and 8th day after treatment, 70 keV single energy CT value and iodine concentration of experimental group were lower than those of control group (t=-3.56, -6.42 and -6.43, -13.01, all P<0.05). There was no significant difference of the percentage of positive cells between 2 groups on day 0 (P=0.11), while the percentage of positive cells in experimental group (33.93%, 25.00%) on the 4th and 8th day were all lower than those in control group (53.28%, 63.80%, both P<0.05). The 70 keV single energy CT values and iodine concentrations of 2 group were all positively correlated with VEGF at different time points (All P<0.05). Conclusion: Energy spectral multi-parameter CT can be used to evaluate the efficacy of Bevacizumab against angiogenesis of C6 glioma rats, which is expected to be a new method for evaluating efficacy of anti-tumor angiogenesis drugs.
