Experimental study on the effect of curcumin on endometriosis in rats
10.12092/j.issn.1009-2501.2020.08.004
- VernacularTitle: 姜黄素对大鼠子宫内膜异位症影响的实验研究
- Author:
Longde SHEN
1
Author Information
1. Obstetrics and Gynecology Department, Quanzhou First Hospital Affiliated to Fujian Medical University
- Publication Type:Journal Article
- Keywords:
Curcumin;
E-cadherin;
Endometriosis;
Syndecan-1;
Twist1;
Twist2
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2020;25(8):862-868
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To analyze the effect of curcumin on the expression of transcription factors Twist1, Twist2, E-cadherin and Syndecan-1 in rats with endometriosis. METHODS: Thirty SD rats were randomly divided into five groups: model group, low dose curcumin group (25 mg•kg-1•d-1), medium dose curcumin group (50 mg•kg-1•d-1), high dose curcumin group (100 mg•kg-1•d-1) and gestrinone group (0.5 mg•kg-1•d-1). There were 6 rats in each group, and 5 rats in sham operation group were set up as blank control group. The expression of Twist1, Twist2, E-cadherin and Syndecan-1 was detected by immunohistochemistry. RESULTS: Compared with the model group, the ectopic tissue volume of curcumin group and gestrinone group was significantly lower, the difference was statistically significant (P<0.05). Positive Twist1 was mainly located in the nucleus, positive Twist2 was mainly located in the cytoplasm, positive E-cadherin and Syndecan-1 staining was mainly located in the epithelial cell membrane, rarely in individual cytoplasm. Compared with the model group, the scores of Twist1, Twist2 and Syndecan-1 in each dose group of curcumin and gestrinone group were significantly lower, and the scores of Twist1, Twist2 and Syndecan-1 in low and middle dose curcumin group were significantly higher than those in gestrinone group, and the scores of E-cadherin were lower than those in gestrinone group (P<0.05). The scores of Twist1, Twist2, E-cadherin and Syndecan-1 in high dose curcumin group were significantly higher. There was no significant difference between the high dose curcumin group and gestrinone group (P>0.05). CONCLUSION: Curcumin can reduce the expression of Twist1, Twist2 and Syndecan-1, increase the expression of E-cadherin, and inhibit the growth of endometrium in the model rats, so as to achieve the purpose of treatment.
