ISSR analysis for genetic diversity of Polygala tenuifolia in its main production area
- Author:
Jia LI
1
Author Information
1. Key Laboratory of Resource Biology and Biotechnology in Western China
- Publication Type:Journal Article
- Keywords:
Genetic diversity;
Germplasm resources;
ISSR;
Polygala tenuifolia Willd.
- From:
Chinese Traditional and Herbal Drugs
2010;41(11):1881-1885
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To discuss the genetic diversity of Polygala tenuifolia in its main production area. Methods: The genetic diversity of ten populations of P. tenuifolia from its main production area Shaanxi, Shanxi, and Hebei Provinces, was analyzed using ISSR markers. Results: Twelve selected ISSR primers generated a total of 276 fragments, 271 of which were polymorphic fragments. The cluster analysis showed that wild and cultivated populations from the same geographical area were clustered first. Genetic variation of both wild and cultivated populations occurred mainly among populations, and the genetic diversity of cultivated populations was lower than wild ones, but both of them were not consistent with the isolation-by-distance model. Conclusion: The results of cluster analysis agree with the fact that cultivated populations are derived directly from wild ones nearby. It was its own reproductive biological features and the current extensive cultivation that resulted in the genetic pattern of P. tenuifolia. Such analyses can offer necessary molecular biological reasons for the utilization and seed breeding of P. tenuifolia.
