Rapid preparation of timosaponin B II reference substance
- Author:
Xu JI
1
Author Information
1. Key Laboratory of Resource Biology and Biotechnology in Western China
- Publication Type:Journal Article
- Keywords:
Preparative HPLC;
Purification;
Separation;
Timosaponin B II
- From:
Chinese Traditional and Herbal Drugs
2010;41(11):1803-1806
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a method to isolate and prepare timosaponin B II reference substance from Anemarrhenae Rhizoma. Methods: Raw material of Anemarrhenae Rhizoma was extracted with 80% ethanol. The concentrated crude extract was separated on a D-101 macroporous resin column, by eluting the macroporous resin column with 30% ethanol, total saponins were collected. The timosaponin B II was separated by preparative reversed-phase high performance liquid chromatography (RP-HPLC) coupled with ELSD and collected according to the chromatography. Results: The prepared product was identified by nuclear magnetic resonance and chemical method. The purity of timosaponin B II was > 99% assayed by analytical HPLC-ELSD. Conclusion: The preparation method is simple and rapid, and it can be used for preparation of timosaponin B TJ reference substance.
