Isolation and determination of biflavonoids in Ginkgo biloba leaves
10.7501/j.issn.0253-2670.2014.17.024
- Author:
Bing LI
1
Author Information
1. School of Traditional Chinese Material Medica, Shenyang Pharmaceutical University
- Publication Type:Journal Article
- Keywords:
Biflavonoids;
Bilobetin;
Ginkgetin;
Ginkgo biloba leaves;
HPLC;
Isoginkgetin;
Sciadopitysin
- From:
Chinese Traditional and Herbal Drugs
2014;45(17):2552-2555
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To purify the biflavonoids from Ginkgo biloba leaves and determine their contents in the leaves collected in different periods. Methods: Dichloromethane fraction of ethanol extract was used to purify biflavonoids through silica gel column and semi-preparative HPLC. The contents of four biflavonoids were simultaneously determined using RP-HPLC. Results: According to the reported 1H-NMR data, four compounds were identified as bilobetin (LB-1), ginkgetin (LB-2), isoginkgetin (LB-3), and sciadopitysin (LB-4). Content determination demonstrated that the biflavonoids accumulation decreased as the leaves collected from the beginning to the end of September, and the content difference can be as large as 30%. Conclusion: RP-HPLC method can be used in the quantitative determination of biflavonoids in G. biloba leaves. The semi-preparative condition can be used in the purification of biflavonoids from G. biloba leaves. The contents of four biflavonoids could be decreased significantly when the leaves turned yellow, which provides the useful information for the collection of G. biloba leaves. However, the mechanism and the accumulation of other kinds of compounds need further study.
