Research on tissue culture and plant regeneration for roots of Ficus hirta
10.7501/j.issn.0253-2670.2014.17.023
- Author:
Lin-Xuan LI
1
Author Information
1. Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement, Guangxi Botanical Garden of Medicinal Plant
- Publication Type:Journal Article
- Keywords:
Adventitious bud;
Explant;
Matrix;
Rooting;
Roots of Ficus hirta Vahl;
Tissue culture
- From:
Chinese Traditional and Herbal Drugs
2014;45(17):2547-2551
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To solve the shortage problem of large-scale planting the seedlings in the roots of Ficus hirta by rapid propagation in vitro tissue culture. Methods: The MS and 1/2 MS media were used as basic media, The multi factor combination of plant growth regulator (6-BA, NAA, 2, 4-D, IAA, KT, and IBA) on the seedling subculture and root culture was studied by orthogonal design. Results: The best medium for the adventitious bud induction was MS + 6-BA 1.0 mg/L + NAA 0.3 mg/L, 72 adventitious buds were obtained by differentiation with 20 d induction of explants; The best medium for cluster inducing and subculture was MS + 1.0 mg/L 6-BA + 0.3 mg/L IAA + 0.3 mg/L KT; The best rooting medium was 1/2 MS + 1.0 mg/L IBA + 0.3 mg/L NAA, and the rooting rate was 100%. Being suitably Transplanted to peat-perlite (1:1) matrix, the 30 d survival rate was 93%. Conclusion: The tissue culture for the roots of F. hirta could be used to produce test-tube seedlings for large scale planting.
