ISSR Genetic diversity analysis of Panax japonicus var. major by molecular makers
10.7501/j.issn.0253-2670.2014.17.019
- Author:
Wei WANG
1
Author Information
1. Shaanxi College of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Cluster analysis;
Genetic diversity;
ISSR;
Panax japonicus C. A. Mey. var. major (Burk.) C. Y. Wu et K. M. Feng;
Principal coordinate analysis
- From:
Chinese Traditional and Herbal Drugs
2014;45(17):2525-2529
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To analyze the genetic diversity of Panax japonicus var. major by inter simple sequence repeat (ISSR) molecular makers. Methods: Genetic diversity of 19 samples from main production areas was investigated by ISSR and analyzed by principal coordinate analysis and UPGMA cluster analysis. Results: A total of 181 bands were generated by 13 ISSR primers, among which 166 bands (91.71%) were polymorphic bands (PPB). The coefficient of genetic similarity ranged from 0.60 to 0.83. The results of UPGMA cluster analysis and principal coordinate analysis showed that the genetic diversity of P. japonicus var. major from the same region presented a geographical distribution regularity. Conclusion: The results of ISSR analysis reveals that P. japonicus var. major has a high genetic diversity level and the genetic relationship closely contacts with the geographical location.
