Effects of galangin with different purity on vitiligo mouse model induced by hydroquinone
10.7501/j.issn.0253-2670.2014.16.015
- Author:
Li GAO
1
Author Information
1. Xinjiang Key Laboratory of Biological Reaources and Genetic Enigneering, College of Life Science and Technology, Xinjiang University
- Publication Type:Journal Article
- Keywords:
Basal melanocytes;
Galangin;
Hydroquinone;
Melanin;
Tyrosinase;
Vitiligo
- From:
Chinese Traditional and Herbal Drugs
2014;45(16):2358-2363
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effects of galangin with different purity on vitiligo mouse model induced by hydroquinone. Methods: The vitiligo mouse model was induced by smearing 2.5% hydroquinone at shaving areas on dorsal skin for each mouse. The concentration of tyrosinase (TYR), the content of malondialdehyde (MDA), and the activity of cholinesterase (CHE) in serum were measured spectrophotometrically. The melanin-containing hair follicles, basal melanocytes, and melanin-containing epidermal cells were measured by histological analysis. The expression of the TYR protein in skin of mice was detected by immunohistochemical methods. Results: Experimental results showed that the hair color of mice in model group was whiter than that of mice in control group, and the numbers of melanin-containing follicle, the numbers of basal melanocyte, and melanin-containing epidermal cell in dorsal skin of mice induced by hydroquinone (2.5%) (model group) were significantly decreased compared with the control group. The color of hair in dorsal skin of mice turned black from white after treatment of galangin with different purity. Histological analysis showed that the numbers of melanin-containing hair follicle in shaving areas were found to be increased at all dosages of the galangin groups (P < 0.01, compared with the model group). While the numbers of skin basal layer melanocyte and melanin-containing epidermal cell were significantly increased at the dosage of 4.25 mg/kg of the 90% galangin (P < 0.05, 0.01), and the expression of skin TYR protein was also increased; The concentration of TYR in serum was increased, the content of MDA and the activity of CHE were decreased at 0.425 and 4.250 mg/kg doses of 90% galangin groups (P < 0.05, compared with the model group). The numbers of skin basal layer melanocyte were increased at 4.250 and 42.500 mg/kg doses of 99% galangin groups; the numbers of melanin-containing epidermal cell were increased (P < 0.05), and the activity of CHE was decreased (P < 0.01) at 4.250 mg/kg dose of 99% galangin group; The concentration of TYR in serum was increased at all dosages of 99% galangin groups; The content of MDA was decreased at 0.425 and 4.250 mg/kg doses of 99% galangin groups (P < 0.01). Conclusion: The result shows that galangin with different purity could improve vitiligo syndrome induced by hydroquinone in mice. The mechanism might be related to the concentration of TYR, the expression of TYR protein, the activity of MDA, and the content of CHE. But there are some differences between 90% galangin and 99% galangin in effects of treatment and mechanism. Galangin might become the one of potential candidate drugs for treating vitiligo diseases after more experiments have been carried out.
