Extraction of phenylethanoid glycoside from Cistanche tubulosa and purification technology by macroporous resin
10.7501/j.issn.0253-2670.2014.16.012
- Author:
Yu-Jie WANG
1
Author Information
1. College of Ethnomedicine, Chengdu University of Traditional Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Acteoside;
Cistanche tubulosa (Schenk) Wight;
Echinacoside;
Macroporous resins;
Phenylethanoid glycoside
- From:
Chinese Traditional and Herbal Drugs
2014;45(16):2344-2348
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To optimize the extraction and purification technology of phenylethanoid glycoside from Cistanche tubulosa. Methods: Orthogonal design L9(34) was employed to optimize the extraction conditions by taking the extraction yield of echinacoside and acteoside as indexes. The absorption-desorption characteristics of seven macroporous resins were evaluated, then the best purification conditions were optimized. Results: The optimal extraction conditions were as follows: The air-dried stems of C. tubulosa were powdered and extracted twice with 12-fold 50% ethanol for 1 h each time, temperature was 70℃; The supernatant was concentrated to 5-fold weight of the stems of C. tubulosa. The concentrated liquid was subjected to macroporous resin (HPD750) column and then eluted with deionezed water (3 BV) and 30% ethanol (4 BV), respectively. The 30% ethanol fraction was evaporated under vacuum to give the phenylethanoid glycoside-rich C. tubulosa stem extract. The purity of phenylethanoid glycoside was above 75%. Conclusion: The optimized extraction and purification process is stable, efficient, and suitable for industrial production.
