Optimization on preparation of Saussureae Involucratae Herba extract phospholipid complex by central composite design-response surface method
10.7501/j.issn.0253-2670.2014.16.009
- Author:
Xue-Feng ZHANG
1
Author Information
1. Pharmaceutical Department, Xinjiang Production and Construction Corps General Hospital
- Publication Type:Journal Article
- Keywords:
Central composite design;
Infrared spectroscopic analysis;
Phospholipid complex;
Saussureae Involucratae Herba extract;
X-ray diffraction
- From:
Chinese Traditional and Herbal Drugs
2014;45(16):2326-2332
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To optimize the preparation of Saussureae Involucratae Herba extract (SIHE) phospholipid complex (PC) by the central composite design-response surface method, and to increase the bioavailability of SIHE. Methods: Five main factors including reaction solvent, reaction time, SIHE concentration, reaction temperature, and ratio of reactants on this reaction were investigated. On the basis of single factor test, central composite design was further adopted to arrange experiments in search of a curve fitting model between factors and effects. The optimal formulation was predicted by response surface method and was verified as well. The structure of SIHE-PC was analyzed by infrared spectroscopic (IR) analysis and X-ray diffraction (XRD). Results: The correlation coefficient of second-order quadratic model was high. The optimal process conditions were as follows: The ratio of SIHE and soybean lecithin was 1∶2.5, reaction concentration was 10 mg/mL, reaction time was 2 h, chologenic acid complex molar ratio was 96%, and rutin complex molar ratio was 93%. IR analysis and XRD proved the formation of SIHE-PC. Conclusion: Central composite design-response surface method is successfully used for the optimization on preparation of SIHE-PC, with good predictability and stability.
