Preparation and analysis of antibody against Poria cocos immunomodulatory protein-1
10.7501/j.issn.0253-2670.2014.20.008
- Author:
Hong-Bo LI
1
Author Information
1. Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province, Department of Life Sciences, Huaihua College
- Publication Type:Journal Article
- Keywords:
Affinity chromatography;
Antibody;
Immunomodulatory protein;
Poria cocos (Schw.) Wolf.;
Poria cocos immunomodulatory protein 1;
Protein-G column
- From:
Chinese Traditional and Herbal Drugs
2014;45(20):2912-2916
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To prepare the recombinant Poria cocos immunomodulatory protein-1 (WCFIP1) and its antibody. Methods: The cDNA of WCFIP1 was synthesized, cloned into plasmid and transformed into Escherichia coli expression strain. The recombinant protein of WCFIP1 was expressed, purified, and injected into mice to prepare antiserum. The antibody against WCFIP1 was purified by portein-G column and the specificity was determined by Western blotting. Results: The recombinant protein of WCFIP1 and the specific antibody were prepared. Conclusion: The recombinant protein of WCFIP1 is expressed and purified from E. coli, and the specifity of the prepared antibody is determined.
