Determination of eight components in Lonicerae Japonicae Flos by HPLC
10.7501/j.issn.0253-2670.2014.07.021
- Author:
Miao LI
1
Author Information
1. Jiangsu Kanion Pharmaceutical Co., Ltd.
- Publication Type:Journal Article
- Keywords:
Caffeic acid;
Chlorogenic acid;
Cryptochlorogenic acid;
Lonicerae Japonicae Flos;
Neochlorogenic acid;
Secoxyloganin
- From:
Chinese Traditional and Herbal Drugs
2014;45(7):1006-1010
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish an HPLC method for simultaneously determining eight components such as neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, caffeic acid, isochlorogenic acid A, isochlorogenic acid B, isochlorogenic acid C, and secoxyloganin in Lonicerae Japonicae Flos. Methods: A RP-HPLC method was established with Luna C18 column (250 mm × 4.6 mm, 5 μm) by a gradient elution using methanol (A) and 0.1% phosphoric acid (B): 0-20 min, 12%-30% A; 20-60 min, 30%-50% A; The flow rate was 1.0 mL/min with double wavelength (237 and 324 nm), and the column temperature was 30°C. Results: The eight components was separated to baseline; Each component had a wide linear range and a good linear relationship (r > 0.9998); The recoveries were 98.72% to 102.50%. Conclusion: The method is accurate, sensitive, reliable, and has a good reproducibility. It could be used for the quality control of Lonicerae Japonicae Flos.
