Isolation and screening of endophytic bacteria with antagonistic activity from Schisandra chinensis
10.7501/j.issn.0253-2670.2014.07.019
- Author:
Yan JIN
1
Author Information
1. College of Agronomy, Jilin Agricultural University
- Publication Type:Journal Article
- Keywords:
Antagonistic bacteria;
Antibacterial activity;
Endophytic bacteria;
Schisandra chinensis (Turcz.) Baill;
Screening
- From:
Chinese Traditional and Herbal Drugs
2014;45(7):996-1001
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To isolate the endophytic bacteria from the plants in Schisandra chinensis. collected from different regions, and to study the flora density, antagonistic bacteria screening, and antibacterial activity. Methods: The endophytic bacteria were isolated by plate isolation method, and to purify their typical strains. Their antagonistic activity in vitro and antibacterial activity were tested by plate confrontation method and growth rate method, respectively. Results: The difference in the flora density of endophytic bacteria in the wild cultivars of S. chinensis was significantly higher than that in the cultured cultivars. Among the parts of S. chinensis, the density of endophytic bacteria was the highest in the roots, followed by the stems, and the lowest in the leaves. Endophytic bacteria (302 strains) were obtained from the isolated bacteria by purification, and the ratio of antagonistic bacteria was 24.19%. Twelve strains were selected with antagonistic action against the pathogens of Fusarium oxysporum, Fusarium Solani, Fusarium oxysporum, Botrytis cirerea, and Alternaria panax, and the fermention of them had inhibitory effect on inhibiting mycelial growth of the tested pathogens. The inhibitory rate of JYg-2 to A. panax was the highest with 80.02%. Conclusion: Plenty of endophytic bacteria are found in healthy plant of S. chinensis and some of them have stable antagonistic activity. Endophytic bacteria of S. chinensis have the great potential on developing biocontrol bacteria.
